Recombinant Bovine Enterokinase


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Recombinant Bovine Enterokinase

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Cat. No. : PRSS7-25B
Product Overview : Recombinant Bovine Enterokinase produced in E.coli is a single glycosylated polypeptide chain containing 235 amino acids and having a molecular mass of approximately28 kDa.
Description : Enterokinase is a serine proteinase in the duodenum that plays a critical role in mammalian digestion. It converts trypsinogen into its active form trypsin, by cleaving its aminoterminal hexapeptide Val(Asp)4-Lys. More recently, the enterokinase has been shown to have a broad utility in cleaving fusion proteins produced in Escherichia coli. The enzyme is particularly suitable for this role because of its high degree of specificity, its tolerance to a wide range of reaction conditions, and the fact that its recognition sequence lies entirely on the aminoterminal side of the scissile bond. This enzymatic activity allows release of carboxyl-terminal fusion partners from fusion proteins without leaving unwanted amino acid residues on their amino termini.
Molecular Weigh : Approximately 28 kDa, a single non-glycosylated polypeptide chain containing 235 amino acids.
Specific activity : ≥ 2 x 104 units/mg recombinant enterokinase.
Unit Definition : One unit is defined as the amount of enzyme needed to cleave 50 ug of fusion protein in 16 hours to 95% completion at 25°C in a buffer containing 25 mM Tris-HCl, pH 7.6, 50 mM NaCl, and 2 mM CaCl2.
Physical Appearance : Sterile liquid.
Formulation : 50 mM Tris-HCl, pH 8.0, 0.5M NaCl and 50% glycerol.
Endotoxin : Less than 1EU/g of rbEK as determined by LAL method.
Storage : One year when stored at -20°C. Avoid repeated freeze/thaw cycles.
Crystal structure of Enteropeptidase with an inhibitorprotease, serine, 7 (enteropeptidase) :

Gene Name : PRSS7 protease, serine, 7 (enterokinase) [ Bos taurus ]
Synonyms : PRSS7; EC; ENTK; Enterokinase; MGC133046; enterokinase; enteropeptidase; proenterokinase; Enteropeptidase precursor; Serine protease 7; human enterokinase;EC; protease, serine, 7; serine protease (enterokinase)
Gene ID : 282009
mRNA Refseq : NM_174439
Protein Refseq : NP_776864
UniProt ID : P98072
Function : peptidase activity; scavenger receptor activity; serine-type endopeptidase activity
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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