Recombinant Bovine Enterokinase PRSS7-25B

Recombinant Bovine Enterokinase

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Recombinant Bovine Enterokinase

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Cat. No. : PRSS7-25B
Product Overview : Recombinant Bovine Enterokinase produced in E.coli is a single glycosylated polypeptide chain containing 235 amino acids and having a molecular mass of approximately28 kDa.
Description : Enterokinase is a serine proteinase in the duodenum that plays a critical role in mammalian digestion. It converts trypsinogen into its active form trypsin, by cleaving its aminoterminal hexapeptide Val(Asp)4-Lys. More recently, the enterokinase has been shown to have a broad utility in cleaving fusion proteins produced in Escherichia coli. The enzyme is particularly suitable for this role because of its high degree of specificity, its tolerance to a wide range of reaction conditions, and the fact that its recognition sequence lies entirely on the aminoterminal side of the scissile bond. This enzymatic activity allows release of carboxyl-terminal fusion partners from fusion proteins without leaving unwanted amino acid residues on their amino termini.
Molecular Weigh : Approximately 28 kDa, a single non-glycosylated polypeptide chain containing 235 amino acids.
Specific activity : ≥ 2 x 104 units/mg recombinant enterokinase.
Unit Definition : One unit is defined as the amount of enzyme needed to cleave 50 ug of fusion protein in 16 hours to 95% completion at 25°C in a buffer containing 25 mM Tris-HCl, pH 7.6, 50 mM NaCl, and 2 mM CaCl2.
Physical Appearance : Sterile liquid.
Formulation : 50 mM Tris-HCl, pH 8.0, 0.5M NaCl and 50% glycerol.
Endotoxin : Less than 1EU/g of rbEK as determined by LAL method.
Storage : One year when stored at -20°C. Avoid repeated freeze/thaw cycles.
Crystal structure of Enteropeptidase with an inhibitorprotease, serine, 7 (enteropeptidase) :

Gene Name : PRSS7 protease, serine, 7 (enterokinase) [ Bos taurus ]
Synonyms : PRSS7; EC 3.4.21.9; ENTK; Enterokinase; MGC133046; enterokinase; enteropeptidase; proenterokinase; Enteropeptidase precursor; Serine protease 7; human enterokinase;EC 3.4.21.9; protease, serine, 7; serine protease (enterokinase)
Gene ID : 282009
mRNA Refseq : NM_174439
Protein Refseq : NP_776864
UniProt ID : P98072
Function : peptidase activity; scavenger receptor activity; serine-type endopeptidase activity

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