Recombinant Human Recoverin

• Cat.No.: RCVRN-191H
• Product Overview: Recombinant Human Recoverin produced inE.Coliis a single, non-glycosylated polypeptide chain. The protein contains 200 amino acids and has a molecular mass of 23 kDa.

Specification

• Cat. No.: RCVRN-191H
• Description: Recoverin is a heterogeneously acylated calcium-binding and intracellular signal transduction protein in the photoreceptor cells of retina. Recoverin contains four EF-hands, of which two bind Ca. Ca-induced extrusion of the acyl group from a hydrophobic cleft in the protein drives the translocation of recoverin from solution to the disc membrane. Recently, recoverin is a detectable serologic protein that is expressed in patients with cancer-associated retinopathy, a paraneoplastic syndrome.
• Source: E.Coli.
• Amino Acid Sequence: MGNSKSGALS KEILEELQLN TKFSEEELCS WYQSFLKDCP TGRITQQQFQ SIYAKFFPDT DPKAYAQHVF RSFDSNLDGT LDFKEYVIAL HMTTAGKTNQ KLEWAFSLYD VDGNGTISKNEVLEIVMAIF KMITPEDVKL LPDDENTPEK RAEKIWKYFG KNDDDKLTEK EFIEGTLANK EILRLIQFEP QKVKEKMKNA.
• Physical Appearance: Sterile filtered clorless solution.
• Formulation: The protein (1mg/ml) contains 20 mM Tris pH 8.0, 1 mM EDTA, 2mM MgCl₂and 10% Glycerol.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Gene Information

• Gene Name: RCVRN recoverin [ Homo sapiens ]
• Synonyms: RCVRN; RCV1; recoverin; RCV-1; Cancer-associated retinopathy protein; Protein CAR; cancer associated retinopathy antigen
• Gene ID: 5957
• mRNA Refseq: NM_002903
• Protein Refseq: NP_002894
• MIM: 179618
• UniProt ID: P35243
• Chromosome Location: 17p13
• Function: calcium ion binding; calcium sensitive guanylate cyclase activator activity