||Recombinant Human actin, gamma 1 produced inE.Colicontains T7 tag at N-terminus and is purified by proprietary chromatographic techniques.
||Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells. Recombinant Human ACTG-1 produced in E.Coli is a non-glycosylated, polypeptide chain containing amino acids (1-375) and having a total molecular mass of 41.8 kda.
||Greater than 95.0% as determined by the analysis by reducing and non-reducing SDS-PAGE coomassie blue staining.
||Sterile Filtered clear solution.
||Recombinant Human Actin Gamma 1 at a concentration of 2mg/ml in 10mM Tris, pH 8.0, 0.1% Triton X-100 and 0.002% NaN3.
||• ELISA • MS • Inhibition Assays • Western Blotting.
||rhACTG-1 although stable at 14°Cfor 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.