||Recombinant Human LA/SS-B produced inSF9is a single, glycosylated, polypeptide chain having a calculated molecular mass of 47.6 kDa, is fused to a hexahistidine purification tag. The LA/SS-B is purified by proprietary chromatographic techniques.
||La is involved in diverse aspects of RNA metabolism, including binding and protecting 3-prime UUU(OH) elements of newly RNA polymerase III (see MIM 606007)-transcribed RNA, processing 5-prime and 3-prime ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. La protein was originally defined by its reactivity with autoantibodies from patients with Sjogren syndrome (MIM 270150) and systemic lupus erythematosus.
||Sf9 insect cells.
||Sterile Filtered clear solution.
||The protein solution contains 20mM HEPES, pH 7.5, 400mM NaCl, 20% Glycerol.
||Greater than 95.0% as determined by SDS-PAGE
||1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checker-board analysis of positive/negative sera panels, immuno-dot test).
||0.6-1.1 µg/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination.
||Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.