Recombinant Human Epidermal Growth Factor 21-Leu

Cat. No.: EGF-166H
Product Overview: Recombinant human EGF 21-Leu produced inE.Coliis a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 6205 Dalton. The recombinant EGF 21-Leu is purified by proprietary chromatographic techniques.
Description: Epidermal growth factor or EGF is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds.
Source: Escherichia Coli.
AA Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Ser-Asp-Ser-Glu.
Supplied: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE gel.
Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with no additives.
Solubility: It is recommended to reconstitute the lyophilized human EGF 21-Leu in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions
Biological Activity: Feldan Bio"s human EGF is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation of MDCK cells is < 10 ng/ml.
Storage: Lyophilized recombinant EGF 21-Leu although stable at room temperature for 3 weeks, should be stored desiccated below -180°C. Upon reconstitution human EGF 21-Leu should be stored at 40°Cbetween 2-7 days and for future use below -180°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Gene Name: EGF epidermal growth factor (beta-urogastrone) [ Homo sapiens ]
Synonyms: EC; ERBB; ERBB1; URG; HOMG4; EGF; HER1; PIG61; Mena; epidermal growth factor; beta-urogastrone; epidermal growth factor (beta-urogastrone); Receptor tyrosine-protein kinase ErbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene; homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth factor receptor epidermal growth factor receptor (avian erythroblastic; leukemia viral (v-erb-b) oncogene homolog) epidermal growth factor receptor (erythroblastic leukemia; viral (v-erb-b) oncogene homolog, avian)
Gene ID: 1950
mRNA Refseq: NM_001963
Protein Refseq: NP_001954
MIM: 611718
Pathway: Bladder cancer; Cytokine-cytokine receptor interaction; Endocytosis; Endometrial cancer; growth factor activity; receptor tyrosine kinase binding; transcription cofactor activity; transmembrane receptor protein tyrosine kinase activator activity; ErbB signaling pathway; Focal adhesion; Gap junction; Glioma; MAPK signaling pathway; Melanoma; Non-small cell lung cancer; Pancreatic cancer; Pathways in cancer; Prostate cancer; Regulation of actin cytoskeleton; Hemostasis; Signaling by EGFR
Function: calcium ion binding; epidermal growth factor receptor binding; growth factor activity; protein binding; transmembrane receptor protein tyrosine kinase activator activity

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.