Recombinant Human Epidermal Growth Factor 21-Leu

Cat. No.: EGF-166H
Product Overview: Recombinant human EGF 21-Leu produced inE.Coliis a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 6205 Dalton. The recombinant EGF 21-Leu is purified by proprietary chromatographic techniques.
Description: Epidermal growth factor or EGF is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds.
Source: Escherichia Coli.
AA Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Ser-Asp-Ser-Glu.
Supplied: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE gel.
Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with no additives.
Solubility: It is recommended to reconstitute the lyophilized human EGF 21-Leu in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions
Biological Activity: Feldan Bio"s human EGF is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation of MDCK cells is < 10 ng/ml.
Storage: Lyophilized recombinant EGF 21-Leu although stable at room temperature for 3 weeks, should be stored desiccated below -180°C. Upon reconstitution human EGF 21-Leu should be stored at 40°Cbetween 2-7 days and for future use below -180°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Gene Name: EGF epidermal growth factor (beta-urogastrone) [ Homo sapiens ]
Synonyms: EC; ERBB; ERBB1; URG; HOMG4; EGF; HER1; PIG61; Mena; epidermal growth factor; beta-urogastrone; epidermal growth factor (beta-urogastrone); Receptor tyrosine-protein kinase ErbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene; homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth factor receptor epidermal growth factor receptor (avian erythroblastic; leukemia viral (v-erb-b) oncogene homolog) epidermal growth factor receptor (erythroblastic leukemia; viral (v-erb-b) oncogene homolog, avian)
Gene ID: 1950
mRNA Refseq: NM_001963
Protein Refseq: NP_001954
MIM: 611718
Pathway: Bladder cancer; Cytokine-cytokine receptor interaction; Endocytosis; Endometrial cancer; growth factor activity; receptor tyrosine kinase binding; transcription cofactor activity; transmembrane receptor protein tyrosine kinase activator activity; ErbB signaling pathway; Focal adhesion; Gap junction; Glioma; MAPK signaling pathway; Melanoma; Non-small cell lung cancer; Pancreatic cancer; Pathways in cancer; Prostate cancer; Regulation of actin cytoskeleton; Hemostasis; Signaling by EGFR
Function: calcium ion binding; epidermal growth factor receptor binding; growth factor activity; protein binding; transmembrane receptor protein tyrosine kinase activator activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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