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Recombinant Mouse Endoglin

Cat. No.: Eng-982M
Product Overview: Recombinant Mouse Endoglin encoding the extracellular domain of mouse Endoglin (Met 1 - Gly 581) was expressed ininsect cells. Mouse Endoglin is a disulfide-linked homodimeric protein. Based on N-terminal sequence analysis, the primary structure of recombinant mature Endoglin starts at Glu 26. Endoglin has a calculated monomeric molecular mass of 61 kDa but as a result of glycosylation, migrates at approximately 75 - 85 kDa under reducing conditions in SDS-PAGE.
Description: Endoglin, also known as CD105, is a Type I integral membrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated, cytoplasmic tail. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, bone marrow pro-erythroblasts, and leukemic cells of lymphoid and myeloid lineages. Human and mouse endoglin share approximately 70% and 97 % amino acid sequence identity in their extracellular and intracellular domains, respectively. In common with betaglycan (also named TßRIII), a proteoglycan that shares regions of sequence similarity, endoglin is an accessory receptor for the TGF- superfamily ligands. Endoglin does not bind ligands by itself, but does so by associating with a ligand-binding serine/threonine kinase receptor. Endoglin binds TGF-1 and TGF-3 but not TGF-2 efficiently by associating with TGF- type II receptor (TßRII). It interacts with activin-A and BMP-7 using either the activin type II or type IIB receptors. In the case of BMP-2 which binds directly to the type I but not the type II BMP receptor, endoglin binds via either BMPR-IA (ALK-3) or BMPR-1B (ALK-6).
Source: Insect cells.
Purity: > 95% (SDS-PAGE and visualized by Silverstain).
Endotoxin level: < 0.1 ng per μg of sCD105.
Stabilizer: None.
Formulation: Lyophilized.
Biologically Activity: Measured by its ability to bind with TGF- RII/Fc in a functional ELISA. Optimal dilutions should be determined by each laboratory for each application.
Reconstitution: The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.
Stability: Upon reconstitution, this cytokine, in the presence of a carrier protein, can be stored under sterile conditions at -20°Cto -70°Cfor three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Gene Name: Eng endoglin [ Mus musculus ]
Synonyms: Eng; endoglin;CD105; AI528660; AI662476; S-endoglin; OTTMUSP00000012838; transmembrane glycoprotein
Gene ID: 13805
mRNA Refseq: NM_001146348
Protein Refseq: NP_001139820
UniProt ID: Q63961
Chromosome Location: 2 21.4 cM
Function: Binding; protein binding; protein homodimerization activity; transforming growth factor beta binding

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