Cat. No. : |
LTA-260H |
Product Overview : |
Recombinant HumanLymphotoxin Alpha is a protein produced ininsect cellscontaining 172 amino acid residues and has an approximate molecular weight of 19.3 kDa based on SDS-PAGE gel and Mass Spectrometry due to glycosylation. |
Description : |
Lymphotoxin alpha, a member of the tumor necrosis factor family, is a cytokineproduced by lymphocytes. LTA is highly inducible, secreted, and exists as homotrimeric molecule. LTA forms heterotrimers with lymphotoxin-beta which anchors lymphotoxin-alpha to the cell surface. LTA mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. LTA is also involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. |
Source : |
Insect cells. |
Purity : |
> 95% by SDS-PAGE and HPLC analysis. |
Endotoxin Level : |
< 0.1 ng per μg of TNF-beta. |
Stabilizer : |
None. |
Formulation : |
Lyophilized. |
Biologically Activity : |
The ED50was determined by the cytolysis of murine L929 cells in the presence of actinomycin D is < 0.05 ng/ml, corresponding to a specific activity of > 2 x 107 units/mg. |
Reconstitution : |
The human TNF-beta should be reconstituted in water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted in water or other buffer solutions or stored at -20°C. |
Stability : |
The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted human TNF-beta should be stored in working aliquots at -20℃. Avoid repeated freeze-thaw cycles. |