Active Recombinant Human Retinoblastoma 1
Cat.No. : | RB1-1192H |
- Specification
- Gene Information
- Related Products
Cat. No. : | RB1-1192H |
Description : | PRB, the product of retinoblastoma susceptibility gene RB-1, is one of the best-studied tumor suppressor gene products. At least two other pRB-related proteins, p107 and p130, have been identified and characterized. Mutations in RB-1 gene are often associated with the occurrence of various tumors. The activity of pRB is regulated through phosphorylation in a cell cycle-dependent manner. The hyperphosphorylated RB protein usually associates with the cell nucleus and binds transcription factors of E2F family. pRB represses transcription of its target genes, such as cdc2, cyclin A, and oncogene c-myc, c-fos through the binding with E2F factors and thereby regulating cell proliferation. The wild type pRB (residue1-928) is expressed in baculovirus system and purified by an affinity column in combination with FPLC chromatography. Purified protein is greater than 95% homogeneous and contains no detectable proteases, DNase, and RNase activity. |
Source : | Sf9 insect cells. |
Usage : | For in vitro use only. |
Application : | Recombinant pRB protein can be used for (1) in vitro function studies including transcription and DNA binding assays, (2) protein-protein interaction assay and (3) cell growth and proliferation assays. |
Form : | Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT and 20% glycerol. |
Activity : | 1 ng is sufficient for a gel mobility shift assay in a 20μl reaction, 50 ng are sufficient for reconstituted transcription assays and 100 ng are sufficient for a protein-protein interaction assay. |
Purity : | > 95% by SDS-PAGE. |
Storage : | Quality guaranteed for 12 months, store at -80℃. Avoid freeze / thaw cycles. |
Gene Name : | RB1 retinoblastoma 1 [ Homo sapiens ] |
Synonyms : | RB1; retinoblastoma 1; RB; pRb; OSRC; pp110; p105-Rb; RB1; osteosarcoma; OTTHUMP00000018397; Rb; retinoblastoma suspectibility protein; retinoblastoma-associated protein |
Gene ID : | 5925 |
mRNA Refseq : | NM_000321 |
Protein Refseq : | NP_000312 |
MIM : | 180200 |
UniProt ID : | P06400 |
Chromosome Location : | 13q14.2 |
Pathway : | Bladder cancer; Cell cycle; Chronic myeloid leukemia; Glioma; Melanoma; Non-small cell lung cancer; Pancreatic cancer; Pathways in cancer; Prostate cancer; Small cell lung cancer; Cell Cycle, Mitotic; DNA Replication |
Function : | androgen receptor binding; kinase binding; phosphoprotein binding; transcription coactivator activity; transcription factor activity; transcription factor binding; transcription repressor activity; ubiquitin protein ligase binding |
Products Types
◆ Recombinant Protein | ||
RB1-3618R | Recombinant Rhesus Macaque RB1 Protein, His (Fc)-Avi-tagged | +Inquiry |
RB1-902H | Recombinant Human RB1 protein, His-GST-tagged | +Inquiry |
RB1-1283R | Recombinant Rat NSF Protein (721-919 aa), His-tagged | +Inquiry |
RB1-4608R | Recombinant Rat RB1 Protein, His (Fc)-Avi-tagged | +Inquiry |
RB1-382H | Recombinant Human RB1 Protein, GST-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
0
Inquiry Basket