Recombinant Human PRKCZ

• Cat.No.: PRKCZ-30179TH
• Product Overview: Recombinant full length PKC zeta protein (Human), was expressed by baculovirus in Sf9 insect cells using a N-terminal tag, MW 93kDa.

Specification

• Description: Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Tissue specificity: Expressed in brain, and to a lesser extent in lung, kidney and testis.
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequence Similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.Contains 1 AGC-kinase C-terminal domain.Contains 1 OPR domain.Contains 1 phorbol-ester/DAG-type zinc finger.Contains 1 protein kinase domain.

Gene Information

• Gene Name: PRKCZ protein kinase C, zeta [ Homo sapiens ]
• Official Symbol: PRKCZ
• Synonyms: PRKCZ; protein kinase C, zeta; protein kinase C zeta type; PKC2
• Gene ID: 5590
• mRNA Refseq: NM_001033581
• Protein Refseq: NP_001028753
• MIM: 176982
• Uniprot ID: Q05513
• Chromosome Location: 1p36.33-p36.2
• Pathway: CDC42 signaling events, organism-specific biosystem; CXCR4-mediated signaling events, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Ceramide signaling pathway, organism-specific biosystem; Chemokine signaling pathway, organism-specific biosystem
• Function: ATP binding; insulin receptor substrate binding; metal ion binding; nucleotide binding; protein binding