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Recombinant Human SMARCA4, His-tagged

Cat.No. : SMARCA4-27675TH
Product Overview : Recombinant full length human BRG1, containing a substitution of lysine to arginine at position 798, expressed in a baculovirus system with His tag.
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Description : The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene.
Source : Baculovirus-insect cell
Species : Human
Tag : His
Form : The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol
Molecular Mass : 189.0 kDa
Purity : The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns. The purified Brg1-mt(K798) is greater than 90% homogeneous based on SDS-PAGE analysis.
Unit Definition : 1 unit equals 1 nanogram purified protein. 50 - 100 units are required for an in vitro nucleosome remodeling assay and 100 units are required for a protein-protein interaction assay.
Applications : Recombinant BRG1 can be used 1) for protein-protein interaction assay; 2) for in vitro transcription assay; 3) for in vitro nucleosome remodeling assay; and 4) for cell growth assay.
Storage : Stored at -70°C before use. Avoid repeated freeze thaw cycles.
Gene Name : SMARCA4 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 [ Homo sapiens (human) ]
Official Symbol : SMARCA4
Synonyms : SMARCA4; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4; BRG1; SNF2; SWI2; BAF190; SNF2L4; SNF2LB; hSNF2b; FLJ39786; SNF2-BETA; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a4; SNF2-like 4; nuclear protein GRB1; brahma protein-like 1; BRM/SWI2-related gene 1; homeotic gene regulator; sucrose nonfermenting-like 4; ATP-dependent helicase SMARCA4; mitotic growth and transcription activator; global transcription activator homologous sequence; SNF2B; Probable global transcription activator SNF2L4; EC 3.6.1.-; BRG-1 protein; nuclear protein GRB1
Gene ID : 6597
mRNA Refseq : NM_003072
Protein Refseq : NP_003063
MIM : 603254
UniProt ID : P51532
Chromosome Location : 19p13.2
Pathway : Chromatin modifying enzymes; Direct p53 effectors; Glucocorticoid receptor regulatory network; Integrated Breast Cancer Pathway
Function : ATP binding; DNA polymerase binding; DNA-dependent ATPase activity; contributes_to RNA polymerase II core promoter proximal region sequence-specific DNA binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Can epigenetic therapies target SMARCA4 alterations? 03/09/2020

Epigenetic therapies that modulate gene expression could indirectly affect SMARCA4-altered cancers, though specific targeting remains a challenge.

Are there diagnostic tests for SMARCA4 mutations? 08/15/2019

Yes, there are genetic tests available to identify SMARCA4 mutations in tumors, aiding in prognosis and treatment decisions.

Are there ongoing clinical trials focusing on SMARCA4 alterations? 01/11/2019

Yes, there are clinical trials exploring new treatment strategies for SMARCA4-altered cancers, aiming to improve patient outcomes.

Is SMARCA4 solely associated with cancer? 04/16/2018

No, SMARCA4's functions extend beyond cancer and have implications in various biological processes, including development and differentiation.

How can SMARCA4 mutations influence patient prognosis? 01/31/2018

Patients with SMARCA4 mutations in their tumors often face a poorer prognosis, which necessitates tailored treatment approaches.

Customer Reviews (3)

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Reviews
02/18/2023

    The SMARCA4 protein offers exceptional quality that meets the rigorous demands of experimental research.

    11/01/2018

      It exhibits exceptional performance in ELISA assays, making it an excellent choice for researchers studying angiopoietins, angiogenesis, or vascular biology.

      10/30/2016

        Its purity, consistency, and reliable performance make it a top choice for a wide range of applications.

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