Expand Expand| Cat. No. : | Sell-1764M |
| Product Overview : | The extracellular doman of mouse L-selectin was fused to the carboxy-terminal 6X his-tagged Fc region of human IgG1 via a polypeptide linker inserted into a suitable mammalian expression vector and expressed inNS0 cells. |
| Description : | L-selectin, also known as CD62L, is a cell adhesion molecule consisting of a large, highly glycosylated, extracellular domain found on leukocytes. It belongs to the selectin family of proteins, which recognise sialylated carbohydrate groups. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via the high endothelial venules. L-selectin is an effective marker for separating lymphoid progenitors from myeloid progenitors and hematopoietic stem cells in bone marrow. |
| Molecular Weight : | The predicted molecular weight of Recombinant Mouse L-Selectin is Mr 60.5 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 150 kDa. |
| Source : | NSO Cells. |
| State Of Matter : | Lyophilized. |
| Purity : | >90% by SDS Page and analyzed by silver stain. |
| Formulation : | This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives. |
| Endotoxin : | <0.1 EU/µg as determined by the LAL method. |
| Biological Activity : | The biological activity of Mouse L-Selectin was determined by the ability of the immobilized protein to support the adhesion of LS180 cells. When 1 x 104cells/well are added to L-Selectin coated plates approximately 90 - 100% will adhere for 1 hour incubation at RT. |
| Storage And Stability : | This lyophilized protein is stable for six to twelve months when stored desiccated at -20℃ to -70℃. After aseptic reconstitution, this protein may be stored at 2℃ to 8℃ for one month or at -20℃ to -70℃ in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. |
| Gene Name : | Sell selectin, lymphocyte [ Mus musculus ] |
| Synonyms : | Sell; selectin, lymphocyte; Lnhr; CD62L; Ly-22; Lyam1; Ly-m22; Lyam-1; LECAM-1; AI528707; L-selectin; selectin L |
| Gene ID : | 20343 |
| mRNA Refseq : | NM_001164059 |
| Protein Refseq : | NP_001157531 |
| UniProt ID : | P18337 |
| Chromosome Location : | 86.6 cM |
| Pathway : | Cell adhesion molecules (CAMs) |
| Function : | Binding; protein binding; sugar binding |
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