Species : |
Mouse |
Source : |
Mammalian Cells |
Tag : |
Fc&His |
Description : |
L-selectin, also known as CD62L, is a cell adhesion molecule consisting of a large, highly glycosylated, extracellular domain found on leukocytes. It belongs to the selectin family of proteins, which recognise sialylated carbohydrate groups. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via the high endothelial venules. L-selectin is an effective marker for separating lymphoid progenitors from myeloid progenitors and hematopoietic stem cells in bone marrow. |
Molecular Weight : |
The predicted molecular weight of Recombinant Mouse L-Selectin is Mr 60.5 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 150 kDa. |
State Of Matter : |
Lyophilized. |
Purity : |
>90% by SDS Page and analyzed by silver stain. |
Formulation : |
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives. |
Endotoxin : |
<0.1 EU/µg as determined by the LAL method. |
Biological Activity : |
The biological activity of Mouse L-Selectin was determined by the ability of the immobilized protein to support the adhesion of LS180 cells. When 1 x 104cells/well are added to L-Selectin coated plates approximately 90 - 100% will adhere for 1 hour incubation at RT. |
Storage And Stability : |
This lyophilized protein is stable for six to twelve months when stored desiccated at -20℃ to -70℃. After aseptic reconstitution, this protein may be stored at 2℃ to 8℃ for one month or at -20℃ to -70℃ in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. |