Active Recombinant Human BRAF/p50 Protein, Flag-His-tagged

• Cat.No.: BRAF-550H
• Product Overview: Recombinant Human BRAF (GenBank accession # NM_004333), a.a. 432-725 with N-terminal His-tag, MW=36 kDa, and p50 (GenBank accession # NM_007065), a.a. 2-378(end) with N-terminal FLAG-tag, MW=45 kDa, coexpressed in a Baculovirus infected Sf9 cell expression system. This protein exists in a complex with Cdc37 and p50.

Specification

• Species: Human
• Source: Insect Cells
• Tag: Flag&His
• Protein Length: 432-725;2-378 a.a.
• Description: This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008]
• Bio-activity: <1 pmol/min/μg.
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Stability: >6 months at –80 centigrade. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).
• Concentration: 0.09 mg/ml
• Storage Buffer: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, 10% glycerol, and 3 mM DTT.
• Warning: Avoid freeze/thaw cycles

Gene Information

• Gene Name: BRAF B-Raf proto-oncogene, serine/threonine kinase [ Homo sapiens (human) ]
• Official Symbol: BRAF
• Synonyms: BRAF; B-Raf proto-oncogene, serine/threonine kinase; B-RAF1; RAFB1; cdc37; p50; NS7; B-raf; BRAF1; RAFB1; B-RAF1; serine/threonine-protein kinase B-raf; 94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); B-Raf serine/threonine-protein; murine sarcoma viral (v-raf) oncogene homolog B1; proto-oncogene B-Raf; v-raf murine sarcoma viral oncogene homolog B; v-raf murine sarcoma viral oncogene homolog B1; EC 2.7.11.1
• Gene ID: 673
• mRNA Refseq: NM_004333
• Protein Refseq: NP_004324
• MIM: 164757
• UniProt ID: P15056

Q&As

Please let us confirm whether or not the following kinase products marketed by your company are products applicable to the Cartagena Law in Japan. If the product is used in Japan and falls under the Cartagena Law, it needs to be controlled appropriately.

This protein is not a living modified organism, so it does not apply to the Cartagena Law in Japan. No worries.