Recombinant Human BRAF/p50 Protein, Flag-His-tagged
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| Cat.No. : | BRAF-550H |
| Product Overview : | Recombinant Human BRAF (GenBank accession # NM_004333), a.a. 432-725 with N-terminal His-tag, MW=36 kDa, and p50 (GenBank accession # NM_007065), a.a. 2-378(end) with N-terminal FLAG-tag, MW=45 kDa, coexpressed in a Baculovirus infected Sf9 cell expression system. This protein exists in a complex with Cdc37 and p50. |
| Description : | This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008] |
| Source : | Insects cell |
| Species : | Human |
| Tag : | N-terminal His-tag (BRAF), N-terminal FLAG-tag (p50) |
| Bio-activity : | <1 pmol/min/μg. |
| Applications : | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Stability : | >6 months at –80 centigrade. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%). |
| Concentration : | 0.09 mg/ml |
| Storage Buffer : | 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, 10% glycerol, and 3 mM DTT. |
| Warning : | Avoid freeze/thaw cycles |
| Gene Name : | BRAF B-Raf proto-oncogene, serine/threonine kinase [ Homo sapiens (human) ] |
| Official Symbol : | BRAF |
| Synonyms : | BRAF; B-Raf proto-oncogene, serine/threonine kinase; B-RAF1; RAFB1; cdc37; p50; NS7; B-raf; BRAF1; RAFB1; B-RAF1; serine/threonine-protein kinase B-raf; 94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); B-Raf serine/threonine-protein; murine sarcoma viral (v-raf) oncogene homolog B1; proto-oncogene B-Raf; v-raf murine sarcoma viral oncogene homolog B; v-raf murine sarcoma viral oncogene homolog B1; EC 2.7.11.1 |
| Gene ID : | 673 |
| mRNA Refseq : | NM_004333 |
| Protein Refseq : | NP_004324 |
| MIM : | 164757 |
| UniProt ID : | P15056 |
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