Recombinant Human Caspase 5, Apoptosis-related Cysteine Peptidase

• Cat.No.: CASP5-600H
• Product Overview: Recombinant Human CASP5 was produced inE. coli.

Specification

• Cat. No.: CASP5-600H
• Description: Caspase 5 is an enzyme that proteolytically cleaves other proteins at an aspartic acid residue, and belongs to a family of cysteine proteases called caspases. It is an inflammatory caspase, along with caspase 1, caspase 4 and the murine caspase 4 homolog caspase 11, and has a role in the immune system.
• Source: E. coli.
• Purity: ≥95% (SDS-PAGE).
• Formulation: Lyophilized.
• Reconstitution: Reconstitute to 1U/µl with PBS containing 15% glycerol.
• Specific Activity: ~5"000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate WEHD-pNA per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
• Application: In combination with caspase-5 activity assays, the enzyme is useful in screening caspase inhibitors, studying enzyme regulation and kinetics, determining target substrate or as a positive control in caspase-5 activity assays. We recommend using 1 unit per assay for analyzing caspase activity.
• Storage: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.

Gene Information

• Gene Name: CASP5 caspase 5, apoptosis-related cysteine peptidase [ Homo sapiens ]
• Synonyms: CASP5; caspase 5, apoptosis-related cysteine peptidase; ICH-3; MGC141966; ICEREL-III; ICE(rel)III; caspase-5; CASP-5; TY protease; protease TY; ICE(rel)-III; protease ICH-3; EC 3.4.22.58; Caspase-5 subunit p20; Caspase-5 subunit p10; caspase 5, apoptosis-related cysteine protease
• Gene ID: 838
• mRNA Refseq: NM_001136109
• Protein Refseq: NP_001129581
• MIM: 602665
• UniProt ID: P51878
• Chromosome Location: 11q22.2-q22.3
• Pathway: NOD-like receptor signaling pathway
• Function: cysteine-type endopeptidase activity; cysteine-type peptidase activity; peptidase activity; protein binding