Active Recombinant Human PDGFRB protein(Met1-Lys531), His-tagged

• Cat.No.: PDGFRB-2187H
• Product Overview: Recombinant Human PDGFRβ (NP_002600.1) extracellular domain (Met 1-Lys 531) was expressed in HEK293, with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Met1-Lys531
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized PDGFRβ-His at 10 μg/ml (100 μl/well) can bind Cynomolgus PDGFB, The EC50 of Cynomolgus PDGFB is 3-8 ng/ml.
• Molecular Mass: The secreted recombinant human PDGFRβ comprises 510 amino acids and predicts a molecular mass of 57.6 kDa. It migrates as an approximately 95-100 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PDGFRB platelet-derived growth factor receptor, beta polypeptide [ Homo sapiens ]
• Official Symbol: PDGFRB
• Synonyms: PDGFRB; platelet-derived growth factor receptor, beta polypeptide; PDGFR; platelet-derived growth factor receptor beta; CD140b; JTK12; PDGFR1; PDGFR-beta; PDGF-R-beta; CD140 antigen-like family member B; platelet-derived growth factor receptor 1; beta-type platelet-derived growth factor receptor; CD140B; PDGFR-1
• Gene ID: 5159
• mRNA Refseq: NM_002609
• Protein Refseq: NP_002600
• MIM: 173410
• UniProt ID: P09619