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Active Recombinant Mouse Acvr2b protein, His-tagged

Cat.No. : Acvr2b-424M
Product Overview : Recombinant Mouse Acvr2b protein(NP_031423.1)(Met1-Thr134), fused with His tag, was expressed in HEK293.
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Source : HEK293
Species : Mouse
Tag : His
Form : Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Bio-activity : 1. Measured by its binding ability in a functional ELISA. Immobilized Mouse ACVR2B at 2μg/mL (100 μl/well) can bind biotinylated mouse INHBA-His, The EC50 of biotinylated mouse INHBA-His is 40-95 ng/mL.2. Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 0.2-0.8 µg/mL in the presence of 10 ng/mL recombinant Activin A.
Molecular Mass : The recombinant mouse ACTRIIB/ACVR2B comprises 127 amino acids and has a predicted molecular mass of 14.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmACVR2B is approximately 33-37 kDa due to glycosylation.
Proteinlength : Met1-Thr134
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 94 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : Acvr2b activin receptor IIB [ Mus musculus ]
Official Symbol : Acvr2b
Synonyms : ACVR2B; activin receptor IIB; activin receptor type-2B; ACTR-IIB; activin receptor type IIB; ActRIIB;
Gene ID : 11481
mRNA Refseq : NM_007397
Protein Refseq : NP_031423

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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