Recombinant Human ENG protein(Met1-Gly586), His-tagged

• Cat.No.: ENG-3146H
• Product Overview: Recombinant Human CD105 (NP_001108225.1) (Met 1-Gly 586) was expressed in HEK293, with C-terminal fused polyhistidine tag.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Met1-Gly586
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human CD105 consists of 572 amino acids after removal of the signal peptide and predicts a molecular mass of 62.3 kDa. As a result of glycosylation, rh CD105 migrates as an approximately 80-90 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ENG endoglin [ Homo sapiens ]
• Official Symbol: ENG
• Synonyms: ENG; endoglin; ORW, ORW1, Osler Rendu Weber syndrome 1; CD105; END; HHT1; CD105 antigen; ORW; ORW1; FLJ41744
• Gene ID: 2022
• mRNA Refseq: NM_000118
• Protein Refseq: NP_000109
• MIM: 131195
• UniProt ID: P17813