Recombinant Human LCK, GST tagged
Cat.No. : | LCK-776H |
Product Overview : | Recombinant Human LCK (NP_001036236.1) (Met 1-Pro 509), fused with the GST tag at the N-terminus, was produced in Baculovirus-Insect cells. |
Unit | |
Price | |
Qty |
- Specification
- Gene Information
- Related Products
Source : | Baculovirus-Insect Cells |
Species : | Human |
Tag : | GST |
Form : | Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 0.5mM GSH, 0.1mM EGTA, 0.1mM EDTA, 0.5mM PMSF, 10% glycerol |
Molecular Mass : | The recombinant human LCK/GST chimera consists of 734 amino acids and predicts a molecular mass of 84.4 kDa. It migrates as an approximately 80 kDa band in SDS-PAGE under reducing conditions. |
Endotoxin : | < 1.0 eu per μg of the protein as determined by the LAL method. |
Stability : | Samples are stable for up to twelve months from date of receipt at -70oC. |
Storage : | Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Gene Name : | LCK lymphocyte-specific protein tyrosine kinase [ Homo sapiens ] |
Official Symbol : | LCK |
Gene ID : | 3932 |
mRNA Refseq : | NM_001042771 |
Protein Refseq : | NP_001036236 |
MIM : | 153390 |
UniProt ID : | P06239 |
Products Types
◆ Recombinant Protein | ||
LCK-3021R | Recombinant Rat LCK Protein, His (Fc)-Avi-tagged | +Inquiry |
LCK-174H | Recombinant Human LCK Protein (SH3 Domain), GST tagged | +Inquiry |
Lck-1300M | Recombinant Mouse Lck Protein, MYC/DDK-tagged | +Inquiry |
LCK-220Z | Recombinant Zebrafish LCK | +Inquiry |
LCK-29937TH | Recombinant Human LCK | +Inquiry |
◆ Lysates | ||
LCK-681HCL | Recombinant Human LCK cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (7)
Ask a questionLCK plays a vital role in the development and maturation of T cells in the thymus, especially during the processes of positive and negative selection, ensuring the generation of a functional and self-tolerant T-cell repertoire.
Yes, LCK activity can be modulated by several pharmacological agents, including kinase inhibitors like dasatinib, which have been explored for therapeutic purposes in various diseases.
LCK activation is regulated post-translationally through phosphorylation at specific tyrosine residues, with its activity being modulated by both activating and inhibitory phosphorylations.
LCK is a pivotal Src family kinase that plays a crucial role in initiating T-cell receptor (TCR) signaling by phosphorylating key tyrosines on the CD3 and ζ-chains of the TCR complex.
LCK interacts with other kinases such as ZAP-70 and Fyn in T-cell signaling pathways, orchestrating a cascade of phosphorylation events that lead to T-cell activation.
Dysregulation of LCK has been associated with various conditions, including autoimmune diseases, immunodeficiencies, and certain leukemias.
LCK contributes to TCR complex formation and activation by phosphorylating the immunoreceptor tyrosine-based activation motifs (ITAMs) on the TCR, facilitating the recruitment and activation of ZAP-70 kinase.
Customer Reviews (3)
Write a reviewGood stability, long shelf-life.
Consistent quality across batches.
Pure product, reliable results.
Ask a Question for All LCK Products
Required fields are marked with *
My Review for All LCK Products
Required fields are marked with *
Inquiry Basket