Active Recombinant Mouse Acvr2a protein(Met1-Pro134), His&hFc-tagged

• Cat.No.: Acvr2a-493M
• Product Overview: Recombinant Mouse ACVR2A (NP_031422.3) (Met 1-Pro 134) was expressed in HEK293, fused with the C-terminal polyhistidine-tagged Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His&hFc
• Protein length: Met1-Pro134
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 20-60 ng/mL in the presence of 10 ng/mL recombinant Activin A.
• Molecular Mass: The secreted recombinant mouse ACVR2A/Fc chimera is a disulfide-linked homodimer. The reduced monomer comprises 363 amino acids and has a calculated molecular mass of 41.4 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 55-65 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Acvr2a activin receptor IIA [ Mus musculus ]
• Official Symbol: Acvr2a
• Synonyms: ACVR2A; activin receptor IIA; activin receptor type-2A; ACTR-IIA; activin receptor type IIA; Acvr2; ActrIIa; TactrII
• Gene ID: 11480
• mRNA Refseq: NM_007396
• Protein Refseq: NP_031422