Recombinant Human EPH Receptor A5, GST-His

• Cat.No.: EPHA5-744H
• Product Overview: Recombinant Human EPHA5, C-terminal fragment, amino acids S595-L1037, activated, Nterminal GST-HIS6 fusion protein with a Thrombin and a 3C cleavage site, expressed inSf9insect cells. MW = 80,683 Da.

Specification

• Cat. No.: EPHA5-744H
• Description: EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.
• Source: Baculovirus infected Sf9 cells.
• Purification: GST-Affinity Chromatography.
• Product Identity: EPHA5, Lot 001, was confirmed as EPHA5 by mass spectroscopy LC-ESI-MS/MS.
• Activation: in vitro auto-activation.
• Storage Buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol.
• Concentration: 0.167 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
• Specific Activity: 247 pmol/μg×min.
• Storage: -80°C. Avoid repeated freeze-thaw cycles!

Gene Information

• Gene Name: EPHA5 EPH receptor A5 [ Homo sapiens ]
• Synonyms: EPHA5; EPH receptor A5; CEK7; EHK1; HEK7; TYRO4; ephrin type-A receptor 5; EK7; EHK-1; EPH-like kinase 7; OTTHUMP00000223029; EPH homology kinase 1; Eph homology kinase-1; receptor protein-tyrosine kinase HEK7; tyrosine-protein kinase receptor EHK-1; EC 2.7.10.1
• Gene ID: 2044
• mRNA Refseq: NM_004439
• Protein Refseq: NP_004430
• MIM: 600004
• UniProt ID: P54756
• Chromosome Location: 4q13.1
• Pathway: Axon guidance
• Function: ATP binding; nucleotide binding; protein binding; receptor activity; transferase activity; transmembrane-ephrin receptor activity