Active Recombinant Mouse Fgfr3 protein(Met1-Tyr367), His-tagged

• Cat.No.: Fgfr3-624M
• Product Overview: Recombinant Mouse FGFR3 (NP_032036.2)(Met 1-Tyr 367) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His
• Protein length: Met1-Tyr367
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit FGF acidic dependent proliferation of Balb/c3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.3-4 μg/mL.
• Molecular Mass: The recombinant mouse FGFR3 comprises 358 amino acids and has a calculated molecular mass of 37 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmFGFR3 is approximately 70-80 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Fgfr3 fibroblast growth factor receptor 3 [ Mus musculus ]
• Official Symbol: Fgfr3
• Synonyms: FGFR3; fibroblast growth factor receptor 3; heparin-binding growth factor receptor; FR3; Mfr3; sam3; CD333; Flg-2; HBGFR; Fgfr-3
• Gene ID: 14184
• mRNA Refseq: NM_001163215
• Protein Refseq: NP_001156687