α-Glucosidase Assay Kit

• Cat.No.: Kit-2504
• Product Overview: α-GLUCOSIDASE hydrolyzes the terminal, non-reducing 1,4-linked α-D-glucose residues with release of α-D-glucose. α-Glucosidase is needed by all animals to hydrolyze maltose to glucose for use as a food. Aberrant activities have been implicated in diseases such as diabetes and Pompe disease. Simple, direct and automation-ready procedures for measuring α-glucosidase activity are becoming popular in Research and Drug Discovery. α-Glucosidase Assay Kit is designed to measure α-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-α-D-glucopyranoside that is hydrolyzed specifically by α-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.

Specification

• Tag: Non
• Storage: -20ºC
• Shipping: RT
• Size: 100 tests
• Detection method: OD405nm
• Compatible Sample Types: Biological
• Features & Benefits: High sensitivity and wide linear range. Use 20 μL sample. The detection limit is 2 U/L, linear up to 250 U/L.
  Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of α-glucosidase activity within 20 minutes.
  Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.
• Assay time: 20 min
• Sensitivity: 2 U/L