|Product Overview:||Histone deacetylases (HDACs) play a central role in controlling cell cycle regulation, cell differentiation, and tissue development. Theseproteins have crucial roles in development and physiology, especially in the heart and nervous system. They are also deeply involved in cellular proliferation, cell cycle and apoptosis. HDAC3 is primarily localized in the nucleus, but can also be found in the cytoplasm and at the plasma membrane. The HDAC3 IP & Activity Assay Kit provides an antibody-based method to specifically immunoprecipitate the HDAC3 complex from cells & tissues and to measure HDAC3 activity fluorometrically. HDA3 is immunoprecipitated from cell or nuclear extract(s) using HDAC3 antibody followed by capturing the complex on protein-A/G beads. The immunoprecipitated complex is incubated with the HDAC substrate. Only the deacetylated substrate is cleaved by the Developer to produce a fluorophore, which can be easily analyzed using a fluorescence plate reader.|
|Applications:||Immunoprecipitation of HDAC3 complex from cell & nuclear extract(s)
Measurement of HDAC3 activity of immunoprecipitated complex/purified enzyme
Screening for activators or inhibitors of HDAC3
|Kit Components:||HDAC Assay Buffer; Cell Lysis Buffer; HDAC Substrate; Developer; AFC (7-amino-4-trifluoromethyl coumarin) Standard (1 mM); Rabbit HDAC2 Antibody (0.2 mg/ml); Rabbit IgG (Control Antibody) (0.5 mg/ml); Protein A/G Sepharose Beads; Positive Control (Jurkat Cell Lysate) (125 μg)|
|Detection method:||Fluorescence (Ex/Em=368/442 nm)|
|Features & Benefits:||Simple, fast and convenient.
Only kit on the market for both IP as well as measuring activity of HDAC3.