HDAC3 Immunoprecipitation (IP) & Activity Assay Kit


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HDAC3 Immunoprecipitation (IP) & Activity Assay Kit

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Cat.No. : Kit-2282
Product Overview : Histone deacetylases (HDACs) play a central role in controlling cell cycle regulation, cell differentiation, and tissue development. Theseproteins have crucial roles in development and physiology, especially in the heart and nervous system. They are also deeply involved in cellular proliferation, cell cycle and apoptosis. HDAC3 is primarily localized in the nucleus, but can also be found in the cytoplasm and at the plasma membrane. The HDAC3 IP & Activity Assay Kit provides an antibody-based method to specifically immunoprecipitate the HDAC3 complex from cells & tissues and to measure HDAC3 activity fluorometrically. HDA3 is immunoprecipitated from cell or nuclear extract(s) using HDAC3 antibody followed by capturing the complex on protein-A/G beads. The immunoprecipitated complex is incubated with the HDAC substrate. Only the deacetylated substrate is cleaved by the Developer to produce a fluorophore, which can be easily analyzed using a fluorescence plate reader.
Applications : Immunoprecipitation of HDAC3 complex from cell & nuclear extract(s)
Measurement of HDAC3 activity of immunoprecipitated complex/purified enzyme
Screening for activators or inhibitors of HDAC3
Storage : -20°C
Size : 25 assays
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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