"HSP90α" Related Products

HSP90α (C-Terminal Domain) TR-FRET Kit

Cat.No.: Kit-1747
Size: 384 reactions
Description: The HSP90α (C-Terminal Domain) TR-FRET assay kit is designed to measure the inhibition of HSP90α binding to its protein target PPID (also known as Cyclophilin D or Cyp40) in a homogeneous, 384 reaction format. This assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple. A sample containing terbium-labeled donor, dye-labeled acceptor, HSP90α, substrate, and an inhibitor is incubated for 2 hours. The protein-protein interaction is then assayed by measuring the TR-FRET between the PPID and HSP90α using a fluorescence reader.
Applications: Great for screening small molecular inhibitors for drug discovery and HTS applications.
Storage: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Kit Components: HSP90α (C-Terminal), Biotin Labeled: 10 µg; -80°C PPID, GST-tag: 10 µg; -80°C Tb donor: 20 µl; -20°C Dye-labeled acceptor: 20 µl; -20°C 3x HSP90 TR-FRET Assay Buffer: 4 ml; -20°C White, Nonbinding, low volume, microtiter plate: 1; Room temp.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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