Human epithelial carcinoma cell line A431 Whole Cell Lysate

Cat.No. : A431-068WCY
Product Overview : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
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Description : The epidermoid carcinoma cell line A-431, derived from an 85-year-old female, is one of a series of cell lines established from solid tumors by D.J. Giard, et al.
Source : Skin
Species : Human
Applications : IP, WB
Stability : The lysate is stable for at least one year from date of shipping when stored at -80 centigrade. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20 centigrade for long term storage.
Storage : Store the lysate at -80 centigrade and avoid repeated freeze / thaw cycles.
Concentration : 1 vial of 100 μg whole cell lysate in RIPA buffer.
1 vial of 250 μL 2 x SDS Sample Buffer (4% SDS, 125 mM Tris-HCl pH 6.8, 10 % Glycerol, 0.002 % Bromphenol blue, 100 mM DTT)
Storage Buffer : Modified RIPA buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1 mM EDTA, 1 x Proteinase inhibitor cocktail mix (Sigma) and 1 mM Na3VO4)
Shipping : Shipped on dry ice.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.


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