||L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3- dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion.
||The IDO1 Inhibitor Screening Assay Kit is designed to measure IDO1 enzyme inhibition. The IDO1 Inhibitor Screening Assay Kit is simple to use. Inhibitor and enzyme are added to a sample containing L-Trp substrate. After a room temperature incubation, activity is determined by measuring the absorption of reaction product at λ=321 nm.
||Useful for the study of IDO1 enzymology, screening inhibitors, and selectivity profiling.
||Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
||IDO1 His-Tag: 40 µg; -80°C IDO1 Reaction Solution: 2 x 10 ml; -80°C 1x IDO1 Assay Buffer: 1 ml; -80°C UV transparent 96-well plate: 1; Room Temp.