||L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3- dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion.
||The IDO2 Inhibitor Screening Assay Kit is designed to measure IDO2 enzyme inhibition. The IDO2 Inhibitor Screening Assay Kit is simple to use. Inhibitor and enzyme are added to a sample containing L-Trp substrate.
||Useful for the study of IDO2 enzymology, screening inhibitors, and selectivity profiling.
||At least 6 months from date of receipt when stored as directed.
||IDO2 His-Tag: 400 µg; -80°C IDO2 Reaction Solution: 2 x 10 ml; -80°C 1x IDO2 Assay Buffer: 1 ml; -80°C UV transparent 96-well plate: 1; Room Temp.