|Product Overview :
||JMJD2B is a JmjC-domain protein that exhibits demethylation activity toward H3-K9Me3 and H3-K36Me3.
||The JMJD2B Homogeneous Assay Kit is designed to measure activity of the JMJD2B for screening and profiling applications, using AlphaLISA technology. The key to the JMJD2B Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD2B enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
||Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
||12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
|Kit Components :
||JMJD2B (KDM4B), GST-tag: 60 µg; -80°C Primary antibody 5: 2 x 12.5 μl; -80°C Biotinylated histone H3 peptide substrate (JMJD2B): 400 μl; -80°C 4x JMJD2B Assay Buffer: 2 ml; -20°C 4x JMJ assay buffer 2 (Incomplete buffer): 1 ml; -20°C 4x Detection buffer: 2 ml; -20°C