||JMJD3 is a JmjC-domain protein that exhibits demethylation activity toward di- and trimethyl-lysine 27 (H3K27me2/3) on histone H3.
||The JMJD3 Homogeneous Assay Kit is designed to measure activity of the JMJD3 for screening and profiling applications, using AlphaLISA technology. The key to the JMJD3 Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD3 enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
||Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
||12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
||JMJD3: 40 µg; -80°C Primary antibody 6: 40 µl; -80°C Biotinylated histone H3 peptide substrate: 500 rxns; -80°C 4x HDM Assay Buffer 3: 2 ml; -20°C 4x HDM Incomplete assay buffer: 1 ml; -20°C 4x Detection buffer: 2 ml; -20°C