MNP

What is MNP?

MNP (Membrane Protein Nanoparticles) is a revolutionary membrane protein display technology developed. It embeds full-length native transmembrane proteins into authentic cell membranes coated on nanoparticle surfaces, perfectly mimicking the physiological protein conformation and lipid microenvironment. Unlike traditional detergent-solubilized proteins, MNP nanoparticles provide a more stable and physiologically relevant membrane protein research tool. Creative BioMart currently offer a series of validated, ready-to-use MNP products targeting key drug targets, with custom development capabilities for virtually any membrane protein of interest.

Core Technology Innovation

Technical Advantage	MNP Nanoparticles	Traditional Soluble Proteins
Conformational Fidelity	⭐⭐⭐⭐⭐ Native membrane environment, intact lipid microdomains	⭐⭐⭐ Detergent disrupts native conformation
Stability	⭐⭐⭐⭐⭐ Nanoparticle protection, stable for weeks at 4°C	⭐⭐ Prone to aggregation and degradation, requires -80°C storage
Bioactivity	⭐⭐⭐⭐⭐ Retains native glycosylation and post-translational modifications	⭐⭐ Incomplete or absent modifications
Application Compatibility	⭐⭐⭐⭐⭐ No detergent required, compatible with cell-based assays	⭐⭐ Detergent interferes with cellular/in vivo experiments
Immunogenicity	⭐⭐⭐⭐⭐ Closer to in vivo antigen presentation	⭐⭐ May expose non-native epitopes

MNP Product Technical Principle

MNP Manufacturing Process Highlights

• Authentic Membrane Extraction: Direct isolation of native membrane components from HEK293 cells, preserving lipid composition and fluidity
• Nanoparticle Coating: Uniform coating of membrane components on biocompatible nanoparticle surfaces (~100-200nm)
• Full-length Protein Embedding: Genetic engineering ensures correct folding and orientation of target membrane proteins in cell membranes
• Detergent-free Process: Complete avoidance of detergent contact, eliminating interference with protein structure

Product Category Navigation

Browse by Target Family:

⭐ GPCR Superfamily

• Class A GPCRs: ADORA2A (Adenosine Receptor), CB1 (Cannabinoid Receptor), CGRPR (Calcitonin Gene-Related Peptide Receptor), TSHR (Thyroid-Stimulating Hormone Receptor)
• Chemokine Receptors: CCR4, CCR7, CCR8
• Somatostatin Receptor: SSTR2

⭐ Ion Channels and Tight Junction Proteins

• CLDN6 (Claudin-6, Tight Junction Protein)
• TM4SF1 (Transmembrane 4 Superfamily Member 1)

⭐ Clinically Important Targets

• CD20 (B-cell Lymphoma Target)
• CD63 (Tetraspanin, Exosome Marker)

MNP Platform Unique Advantages

✅ Maximum Physiological Relevance: Authentic cell membranes coated on nanoparticle surfaces perfectly mimic cell surface microenvironments, ensuring native conformation, oligomeric states, and lipid interactions of membrane proteins

✅ Ready-to-Use Without Optimization: Pre-formed nanoparticles can be directly used in ELISA, FACS, and cell-based assays without complex refolding or detergent screening

✅ Superior Stability: Stable for 4-6 weeks at 4°C, avoiding activity loss from repeated freeze-thaw cycles, inter-batch CV < 10%

✅ Multi-Scenario Application Compatibility:

• Antibody Discovery: As immunogen or screening antigen
• Cell Therapy: CAR-T/scFv affinity validation
• Small Molecule Screening: Binding assays and functional detection
• Vaccine Development: Nanoparticle-enhanced immunogenicity

✅ No Detergent Interference: Suitable for cell-based and in vivo studies that are detergent-sensitive

Quick Start Workflow

Step 1: Target Selection → Browse pre-loaded membrane protein MNPs above.

Step 2: Application Confirmation → Review Resource 2 (Antibody Development) or Resource 5 (CAR-T Applications)

Step 3: Experimental Preparation → Download Resource 7 (Handling Manual) for best practices

Step 4: Data Validation → Reference Resource 3 (Activity Validation) to design control experiments

Step 5: In-Depth Research → Explore Resource 4 (Structural Characterization) or Resource 6 (Technical Principles)