| Description : |
Type I collagen is the most abundant collagen and is found in connective tissues including tendon, ligament, dermis and blood vessel. It is the major component and the primary determinant of tensile strength of the extracelluar matrix (ECM). It is widely used as a thin layer on tissue-culture surfaces to enhance the attachment and proliferation of a variety of cells including endothelial cells, fibroblasts, hepatocytes, epithelial cells etc. In addition, collagen I can self-assemble into a 3-D superamolecular gel in vitro, making it an ideal biological scaffold to promote more in vivo-like cellular morphology and function. |
| Source : |
Mouse tail tendons |
| Species : |
Mouse |
| Form : |
Lyophilized, salt free, 1 mg/vial |
| Purity : |
Mouse collagen type I > 90%
Mouse collagen type III < 10% |
| Applications : |
Cell culture (coating or 3D gel) |
| Storage : |
Collagen dissolved in acetic acid is stable at 4 centigrade for 1 month. Lyophilized collagen long term storage at -20 centigrade or lower. |
| Reconstitution : |
Dissolve in 20 mM acetic acid. Suspension should be shaken or stirred for several hours or overnight between 2-8 centigrade. Material is dissolved when it appears homogeneous, with no schlieren pattern (light refraction due to differing protein concentrations in the suspensions). Recommended concentration 1-5 mg/mL. |
| Official Symbol : |
Collagen Type I |
| Synonyms : |
COL1A1; COL1A2; osteogenesis imperfecta; Collagen Type I |
