Species : |
Mouse |
Source : |
Mouse Tail Tendon |
Description : |
Type I collagen is the most abundant collagen and is found in connective tissues including tendon, ligament, dermis and blood vessel. It is the major component and the primary determinant of tensile strength of the extracelluar matrix (ECM). It is widely used as a thin layer on tissue-culture surfaces to enhance the attachment and proliferation of a variety of cells including endothelial cells, fibroblasts, hepatocytes, epithelial cells etc. In addition, collagen I can self-assemble into a 3-D superamolecular gel in vitro, making it an ideal biological scaffold to promote more in vivo-like cellular morphology and function. |
Form : |
Lyophilized, salt free, 1 mg/vial |
Purity : |
Murine Collagen Type I at least 90% Non- Collagen proteins below 2% |
Applications : |
Used for coating material for cell culture studies. May not be suitable for 3-D gel formation. |
Storage : |
Collagen dissolved in acetic acid is stable at 4 centigrade for 1 month. Lyophilized collagen long term storage (2 years) at -20 centigrade or lower. |
Reconstitution : |
Add 0.01-0.05 M acetic acid to yield 1-5 mg/mL collagen solution. Gentle mixing overnight at 4 centigrade. |