|Product Overview:||This product is the Agarose bound Pisum Sativum Agglutinin (PSA) and has sugar specificity against Mannose and Glucose.|
|Description:||Pisum sativum agglutinin is nearly identical in structure and carbohydrate specificity to Lens culinaris agglutinin. The lectin has specificity toward α-linked mannose-containing oligosaccharides, with an N-acetylchitobiose-linked α-fucose residue included in the receptor sequence. Calcium and manganese ions are required for activity. PSA has been used to fractionate cells, to isolate glycoproteins and glycopeptides, to distinguish between normal and virally transformed cells, as a T-cell mitogen, and as an inhibitor of allograft rejection.|
|Form:||10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM Ca2+, 0.08% sodium azide, 0.1 M glucose|
|Bio-activity:||Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside|
|Molecular Mass:||53 kDa|
|Applications:||Glycobiology, Affinity Chromatography|
|Usage:||1, Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin.
2, Suggested conditions for eluting glycoconjugates: 200 mM α-methyl mannoside/200 mM α-methyl glucoside mixture.
|Storage:||Refrigerate. DO NOT FREEZE.|
|Concentration:||3.0 mg/ml of settled gel|