| Tag : |
Non |
| Size : |
96 assay |
| Applications : |
1) Screening inhibitors of PCSK9-LDLR interaction in vitro.2) Characterization of inhibitor candidates of PCSK9-LDLR interaction in vitro.3) Screening monoclonal antibody that inhibits PCSK9-LDLR interaction in vitro.4) Characterization of PCSK9-LDLR interaction in vitro. |
| Storage : |
Upon receipt, store all other components at 4°C;Don"t expose reagents to excessive light.All of the reagents included in the CycLex Research Product CircuLex PCSK9-LDLR in vitro Binding Assay Kit have been tested for stability. Reagents should not be used beyond the stated expiration date. |
| Kit Components : |
All samples should be assayed in duplicate. The following components are supplied and are sufficient for the one 96-well microtiter plate kit.Microplate: One microplate supplied ready to use, with 96 wells (12 strips of 8-wells) in a foil, zip-lock bag with a desiccant pack. Wells are coated with Recombinant LDL-EGF-AB domain.Recombinant His-tagged PCSK9 wild type: One vial containing 1.2 µg of lyophilized recombinant His-tagged PCSK9 wild type.150X Biotinylated anti-His-tag monoclonal antibody: One vial containing 100 µL of 150X Biotinylated anti-His-tag monoclonal antibody. For long term storage, unused 150X Biotinylated anti-His-tag monoclonal antibody solution can be stored in small aliquots (e.g. 20 µL) at below -70°C to avoid repeated freezing and thawing.Reaction Buffer: One bottle containing 50 mL of 1X buffer; used for binding reaction and dilution of Recombinant His-tagged PCSK9 wild type. Ready to use.150X HRP-conjugated Streptavidin: One vial containing 100 µL of 150X HRP-conjugated Streptavidin. For long term storage, unused 150X HRP-conjugated Streptavidin solution can be stored in small aliquots (e.g. 20 µL) at below -70°C to avoid repeated freezing and thawing.Conjugate Dilution Buffer: One bottle containing 30 mL of 1X buffer; used for dilution of HRP-conjugated Streptavidin. Ready to use10X Wash Buffer: One bottle containing 100 mL of 10X buffer containing 2%Tween-20Substrate Reagent: One bottle containing 20 mL of the chromogenic substrate, tetra methylbenzidine (TMB). Ready to use.Stop Solution: One bottle containing 20 mL of 1 N H2SO4. Ready to use. |
