"PD-1:PD-L1" Related Products

PD-1[Biotinylated]:PD-L1 Homogeneous Assay Kit

Cat.No.: Kit-1816
Size: 384 reactions
Description: The PD-1[Biotinylated]:PD-L1 Homogeneous Assay Kit is designed to measure the inhibition of PD-1 binding to PD-L1. The PD-1[Biotinylated]:PD-L1 Homogeneous Assay Kit comes in a convenient AlphaLISA format with purified biotinylated PD-1, FLAG-tagged PD-L1, and assay buffer to perform a total of 384 reactions. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing biotinylated PD-1 and an inhibitor of choice is incubated with the FLAG-tagged PD-L1 for 60 minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.
Applications: Useful for screening for inhibitors of PD-1 binding to PD-L1
Storage: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Kit Components: PD-1-Biotin: 5 µg; -80°C PD-L1-FLAG: 35 µg; -80°C 3x PD-1 homogeneous assay buffer: 4 ml; -20°C

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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