||Cell signaling through the PD-1 receptor upon binding the PD-L2 ligand attenuates immune responses and is exploited by both tumors and viruses. The PD-1:PDL2[ Biotinylated] Inhibitor Screening Colorimetric Assay Kit is designed for screening inhibitors of PD-L2 ligand binding to PD-1 receptor. The key to this kit is the high affinity of biotin-labeled PD-L2 for streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-1 is coated on a 96-well plate. Next, PD-L2 is incubated with PD-1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.
||This kit is most useful for screening for inhibitors of PD1 binding to PD-L2.
||Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
||PD-L2, Biotin-labeled: 5 µg; -80°C PD-1: 10 µg; -80°C Streptavidin-HRP: 15 µl; +4°C 3x PD-1 assay buffer: 50 ml; -20°C Blocking buffer: 50 ml; +4°C Colorimetric HRP substrate: 10 ml; +4°C Transparent 96-well microplate: 1; +4°C