- 2019-nCoV(12)
- BCoV(2)
- Coronavirus(1)
- COVID-19(94)
- HCoV-229E(1)
- HCoV-EMC/2012(2)
- HCoV-HKU1(1)
- HCoV-NL63(1)
- HCoV-OC43(3)
- Human(1)
- Human coronavirus OC43(1)
- MERS-CoV(2)
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- SARS-CoV-2(10)
Recombinant COVID-19 Spike protein, His-tagged
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| Cat.No. : | S1-137V |
| Product Overview : | Recombinant COVID-19 Spike protein (16-685aa) was fused to His-tag at the N-terminus and expressed in Yeast. |
| Description : | It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
| Source : | Yeast |
| Species : | COVID-19 |
| Tag : | His |
| Form : | Lyophilized powder. Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0. |
| Molecular Mass : | 88.1 kDa |
| AA Sequence : | VNLTTRTQLP PAYTNSFTRG VYYPDKVFRS SVLHSTQDLF LPFFSNVTWF HAIHVSGTNG TKRFDNPVLP FNDGVYFAST EKSNIIRGWI FGTTLDSKTQ SLLIVNNATN VVIKVCEFQF CNDPFLGVYY HKNNKSWMES EFRVYSSANN CTFEYVSQPF LMDLEGKQGN FKNLREFVFK NIDGYFKIYS KHTPINLVRD LPQGFSALEP LVDLPIGINI TRFQTLLALH RSYLTPGDSS SGWTAGAAAY YVGYLQPRTF LLKYNENGTI TDAVDCALDP LSETKCTLKS FTVEKGIYQT SNFRVQPTES IVRFPNITNL CPFGEVFNAT RFASVYAWNR KRISNCVADY SVLYNSASFS TFKCYGVSPT KLNDLCFTNV YADSFVIRGD EVRQIAPGQT GKIADYNYKL PDDFTGCVIA WNSNNLDSKV GGNYNYLYRL FRKSNLKPFE RDISTEIYQA GSTPCNGVEG FNCYFPLQSY GFQPTNGVGY QPYRVVVLSF ELLHAPATVC GPKKSTNLVK NKCVNFNFNG LTGTGVLTES NKKFLPFQQF GRDIADTTDA VRDPQTLEIL DITPCSFGGV SVITPGTNTS NQVAVLYQDV NCTEVPVAIH ADQLTPTWRV YSTGSNVFQT RAGCLIGAEH VNNSYECDIP IGAGICASYQ TQTNSPRRAR |
| Purity : | Greater than 90% as determined by SDS-PAGE. |
| Storage : | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Reconstitution : | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Handling : | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| SDS-PAGE of S1-137V : |
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. |
| Gene Name : | S |
| Official Symbol : | S |
| Synonyms : | spike glycoprotein |
| Gene ID : | 43740568 |
| Protein Refseq : | YP_009724390.1 |
| UniProt ID : | P0DTC2 |
◆ Recombinant Protein
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| Spike-03S | Recombinant 2019-nCoV S1+S2 ECD(L18F, D80A, D215G, 242-244 deletion, R246I, K417N, E484K, N501Y, A701V) Protein, His-tagged | +Inquiry |
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◆ Lysates
| Spike-1058HCL | Recombinant HCoV-EMC/2012 Spike cell lysate | +Inquiry |
| Spike-1741HCL | Recombinant Human coronavirus Spike cell lysate | +Inquiry |
| Spike-001HCL | Recombinant HCoV-EMC/2012 Spike cell lysate | +Inquiry |
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