Recombinant SARS Coronavirus Spike Glycoprotein Mosaic (S2, S2') protein

• Cat.No.: Spike-205V
• Product Overview: Recombinant SARS Coronavirus Spike Glycoprotein Mosaic (S2, S2') protein was expressed in E.coli. Mosaic protein contains C-terminal section of the Spike protein 1051-1076, 1121-1154, 1162-1190 amino acids immunodominant regions.

Specification

• Species: SARS-CoV
• Source: E.coli
• Protein Length: 1051-1076;1121-1154;1162-1190 a.a.
• Description: Spike protein S2: Mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.Spike protein S2': Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.
• Form: Liquid. 25mM Tris-HCl, 0.4% sarcosyl, 0.25% Triton X-100 and 50% glycerol.
• Molecular Mass: 37 kDa
• Purity: >95% as determined by 10% PAGE (coomassie staining).
• Applications: ELISA, Western blot.
• Storage: Upon arrival, storeat below -18 centigrade.
• Shipping: Dry Ice.
• Warning: For Research Use Only.

Gene Information

• Synonyms: sars2; E2; sars3a