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Recombinant Dengue virus E protein, His-tagged

Cat.No.: E-185D
Product Overview: Recombinant Dengue virus(type 2, strain New Guinea C/PUO-218 hybrid) Envelope Protein(Phe247-Gly675) fused with two amino acids "MV" at the N-terminal) and a polyhistidine tag at the C-terminus was expressed in Insect cells.
Description: Envelope played an important role in many functions.
Source: Insect cells
Species: Dengue virus
Tag: His
Predicted N Terminal: Met
Form: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol.
Molecular Mass: The recombinant envelope protein of Dengue virus (DENV)(type 2, strain New Guinea C/PUO-218 hybrid) consists of 442 amino acids and predicts a molecular mass of 49.2 kDa.
Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
Purity: >90 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade
Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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