Recombinant Full Length Human ALK Protein, C-Flag-tagged

• Cat.No.: ALK-1151HFL
• Product Overview: Recombinant Full Length Human ALK Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 176.3 kDa
• AA Sequence: MGAIGLLWLLPLLLSTAAVGSGMGT GQRAGSPAAGPPLQPREPLSYSRLQ RKSLAVDFVVPSLFRVYARD LLLPPSSSELKAGRPEARGSLALDC APLLRLLGPAPGVSWTAGSPAPAEA RTLSRVLKGGSVRKLRRAKQ LVLELGEEAILEGCVGPPGEAAVGL LQFNLSELFSWWIRQGEGRLRIRLM PEKKASEVGREGRLSAAIRA SQPRLLFQIFGTGHSSLESPTNMPS PSPDYFTWNLTWIMKDSFPFLSHRS RYGLECSFDFPCELEYSPPL HDLRNQSWSWRRIPSEEASQMDLLD GPGAERSKEMPRGSFLLLNTSADSK HTILSPWMRSSSEHCTLAVS VHRHLQPSGRYIAQLLPHNEAAREI LLMPTPGKHGWTVLQGRIGRPDNPF RVALEYISSGNRSLSAVDFF ALKNCSEGTSPGSKMALQSSFTCWN GTVLQLGQACDFHQDCAQGEDESQM CRKLPVGFYCNFEDGFCGWT QGTLSPHTPQWQVRTLKDARFQDHQ DHALLLSTTDVPASESATVTSATFP APIKSSPCELRMSWLIRGVL RGNVSLVLVENKTGKEQGRMVWHVA AYEGLSLWQWMVLPLLDVSDRFWLQ MVAWWGQGSRAIVAFDNISI SLDCYLTISGEDKILQNTAPKSRNL FERNPNKELKPGENSPRQTPIFDPT VHWLFTTCGASGPHGPTQAQ CNNAYQNSNLSVEVGSEGPLKGIQI WKVPATDTYSISGYGAAGGKGGKNT MMRSHGVSVLGIFNLEKDDM LYILVGQQGEDACPSTNQLIQKVCI GENNVIEEEIRVNRSVHEWAGGGGG GGGATYVFKMKDGVPVPLII AAGGGGRAYGAKTDTFHPERLENNS SVLGLNGNSGAAGGGGGWNDNTSLL WAGKSLQEGATGGHSCPQAM KKWGWETRGGFGGGGGGCSSGGGGG GYIGGNAASNNDPEMDGEDGVSFIS PLGILYTPALKVMEGHGEVN IKHYLNCSHCEVDECHMDPESHKVI CFCDHGTVLAEDGVSCIVSPTPEPH LPLSLILSVVTSALVAALVL AFSGIMIVYRRKHQELQAMQMELQS PEYKLSKLRTSTIMTDYNPNYCFAG KTSSISDLKEVPRKNITLIR GLGHGAFGEVYEGQVSGMPNDPSPL QVAVKTLPEVCSEQDELDFLMEALI ISKFNHQNIVRCIGVSLQSL PRFILLELMAGGDLKSFLRETRPRP SQPSSLAMLDLLHVARDIACGCQYL EENHFIHRDIAARNCLLTCP GPGRVAKIGDFGMARDIYRASYYRK GGCAMLPVKWMPPEAFMEGIFTSKT DTWSFGVLLWEIFSLGYMPY PSKSNQEVLEFVTSGGRMDPPKNCP GPVYRIMTQCWQHQPEDRPNFAIIL ERIEYCTQDPDVINTALPIE YGPLVEEEEKVPVRPKDPEGVPPLL VSQQAKREEERSPAAPPPLPTTSSG KAAKKPTAAEVSVRVPRGPA VEGGHVNMAFSQSNPPSELHRVHGS RNKPTSLWNPTYGSWFTEKPTKKNN PIAKKEPHERGNLGLEGSCT VPPNVATGRLPGASLLLEPSSLTAN MKEVPLFRLRHFPCGNVNYGYQQQG LPLEAATAPGAGHYEDTILK
  SKNSMNQPGPTRTRPLEQKLISEED LAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase

Gene Information

• Gene Name: ALK ALK receptor tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: ALK
• Synonyms: ALK1; CD246; NBLST3
• Gene ID: 238
• mRNA Refseq: NM_004304.5
• Protein Refseq: NP_004295.2
• MIM: 105590
• UniProt ID: Q9UM73