Recombinant Full Length Human OTUB1 Protein, C-Flag-tagged

• Cat.No.: OTUB1-895HFL
• Product Overview: Recombinant Full Length Human OTUB1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 31.1 kDa
• AA Sequence: MAAEEPQQQKQEPLGSDSEGVNCLA YDEAIMAQQDRIQQEIAVQNPLVSE RLELSVLYKEYAEDDNIYQ QKIKDLHKKYSYIRKTRPDGNCFYR AFGFSHLEALLDDSKELQRFKAVSA KSKEDLVSQGFTEFTIEDF HNTFMDLIEQVEKQTSVADLLASFN DQSTSDYLVVYLRLLTSGYLQRESK FFEHFIEGGRTVKEFCQQE
  VEPMCKESDHIHIIALAQALSVSIQ VEYMDRGEGGTTNPHIFPEGSEPKV YLLYRPGHYDILYKTRTRPLEQKLI SEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Protease

Gene Information

• Gene Name: OTUB1 OTU deubiquitinase, ubiquitin aldehyde binding 1 [ Homo sapiens (human) ]
• Official Symbol: OTUB1
• Synonyms: OTB1; OTU1; HSPC263
• Gene ID: 55611
• mRNA Refseq: NM_017670.3
• Protein Refseq: NP_060140.2
• MIM: 608337
• UniProt ID: Q96FW1