Recombinant Full Length Human PAH Protein, C-Flag-tagged

• Cat.No.: PAH-16HFL
• Product Overview: Recombinant Full Length Human PAH Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the biopterin-dependent aromatic amino acid hydroxylase protein family. The encoded phenylalanine hydroxylase enzyme hydroxylates phenylalanine to tyrosine and is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 51.7 kDa
• AA Sequence: MSTAVLENPGLGRKLSDFGQETSYI EDNCNQNGAISLIFSLKEEVGALAK VLRLFEENDVNLTHIESRPS RLKKDEYEFFTHLDKRSLPALTNII KILRHDIGATVHELSRDKKKDTVPW FPRTIQELDRFANQILSYGA ELDADHPGFKDPVYRARRKQFADIA YNYRHGQPIPRVEYMEEGKKTWGTV FKTLKSLYKTHACYEYNHIF PLLEKYCGFHEDNIPQLEDVSQFLQ TCTGFRLRPVAGLLSSRDFLGGLAF RVFHCTQYIRHGSKPMYTPE PDICHELLGHVPLFSDRSFAQFSQE IGLASLGAPDEYIEKLATIYWFTVE FGLCKQGDSIKAYGAGLLSS FGELQYCLSEKPKLLPLELEKTAIQ NYTVTEFQPLYYVAESFNDAKEKVR NFAATIPRPFSVRYDPYTQR
  IEVLDNTQQLKILADSINSEIGILC SALQKIKTRTRPLEQKLISEEDLAA NDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Phenylalanine, tyrosine and tryptophan biosynthesis, Phenylalanine metabolism

Gene Information

• Gene Name: PAH phenylalanine hydroxylase [ Homo sapiens (human) ]
• Official Symbol: PAH
• Synonyms: PH; PKU; PKU1
• Gene ID: 5053
• mRNA Refseq: NM_000277.3
• Protein Refseq: NP_000268.1
• MIM: 612349
• UniProt ID: P00439