Recombinant Full Length Human UBA7 Protein, C-Flag-tagged

• Cat.No.: UBA7-1673HFL
• Product Overview: Recombinant Full Length Human UBA7 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 111.5 kDa
• AA Sequence: MDALDASKLLDEELYSRQLYVLGSP AMQRIQGARVLVSGLQGLGAEVAKN LVLMGVGSLTLHDPHPTCWS DLAAQFLLSEQDLERSRAEASQELL AQLNRAVQVVVHTGDITEDLLLDFQ VVVLTAAKLEEQLKVGTLCH KHGVCFLAADTRGLVGQLFCDFGED FTVQDPTEAEPLTAAIQHISQGSPG ILTLRKGANTHYFRDGDLVT FSGIEGMVELNDCDPRSIHVREDGS LEIGDTTTFSRYLRGGAITEVKRPK TVRHKSLDTALLQPHVVAQS SQEVHHAHCLHQAFCALHKFQHLHG RPPQPWDPVDAETVVGLARDLEPLK RTEEEPLEEPLDEALVRTVA LSSAGVLSPMVAMLGAVAAQEVLKA ISRKFMPLDQWLYFDALDCLPEDGE LLPSPEDCALRGSRYDGQIA VFGAGFQEKLRRQHYLLVGAGAIGC ELLKVFALVGLGAGNSGGLTVVDMD HIERSNLSRQFLFRSQDVGR PKAEVAAAAARGLNPDLQVIPLTYP LDPTTEHIYGDNFFSRVDGVAAALD SFQARRYVAARCTHYLKPLL EAGTSGTWGSATVFMPHVTEAYRAP ASAAASEDAPYPVCTVRYFPSTAEH TLQWARHEFEELFRLSAETI NHHQQAHTSLADMDEPQTLTLLKPV LGVLRVRPQNWQDCVAWALGHWKLC FHYGIKQLLRHFPPNKVLED GTPFWSGPKQCPQPLEFDTNQDTHL LYVLAAANLYAQMHGLPGSQDWTAL RELLKLLPQPDPQQMAPIFA SNLELASASAEFGPEQQKELNKALE VWSVGPPLKPLMFEKDDDSNFHVDF VVAAASLRCQNYGIPPVNRA QSKRIVGQIIPAIATTTAAVAGLLG LELYKVVSGPRPRSAFRHSYLHLAE NYLIRYMPFAPAIQTFHHLK WTSWDRLKVPAGQPERTLESLLAHL QEQHGLRVRILLHGSALLYAAGWSP EKQAQHLPLRVTELVQQLTG
  QAPAPGQRVLVLELSCEGDDEDTAF PPLHYELTRTRPLEQKLISEEDLAA NDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Parkinson's disease, Ubiquitin mediated proteolysis

Gene Information

• Gene Name: UBA7 ubiquitin like modifier activating enzyme 7 [ Homo sapiens (human) ]
• Official Symbol: UBA7
• Synonyms: D8; UBE2; UBE7; UBA1B; UBE1L
• Gene ID: 7318
• mRNA Refseq: NM_003335.3
• Protein Refseq: NP_003326.2
• MIM: 191325
• UniProt ID: P41226