Recombinant Full Length Human XRCC4 Protein, C-Flag-tagged

• Cat.No.: XRCC4-420HFL
• Product Overview: Recombinant Full Length Human XRCC4 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand breaks. This protein plays a role in both non-homologous end joining and the completion of V(D)J recombination. Mutations in this gene can cause short stature, microcephaly, and endocrine dysfunction (SSMED). Alternate transcript variants such as NM_022406 are unlikely to be expressed in some individuals due to a polymorphism (rs1805377) in the last splice acceptor site.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 37.9 kDa
• AA Sequence: MERKISRIHLVSEPSITHFLQVSWE KTLESGFVITLTDGHSAWTGTVSES EISQEADDMAMEKGKYVGEL RKALLSGAGPADVYTFNFSKESCYF FFEKNLKDVSFRLGSFNLEKVENPA EVIRELICYCLDTIAENQAK NEHLQKENERLLRDWNDVQGRFEKC VSAKEALETDLYKRFILVLNEKKTK IRSLHNKLLNAAQEREKDIK QEGETAICSEMTADRDPVYDESTDE ESENQTDLSGLASAAVSKDDSIISS LDVTDIAPSRKRRQRMQRNL
  GTEPKMAPQENQLQEKENSRPDSSL PETSKKEHISAENMSLETLRNSSPE DLFDEITRTRPLEQKLISEEDLAAN DILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Non-homologous end-joining

Gene Information

• Gene Name: XRCC4 X-ray repair cross complementing 4 [ Homo sapiens (human) ]
• Official Symbol: XRCC4
• Synonyms: SSMED; hXRCC4
• Gene ID: 7518
• mRNA Refseq: NM_022550.4
• Protein Refseq: NP_072044.1
• MIM: 194363
• UniProt ID: Q13426