| Description : |
4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
56.5 kDa |
| AA Sequence : |
MASMLLAQRLACSFQHSYRLLVPGS RHISQAAAKVDVEFDYDGPLMKTEV PGPRSRELMKQLNIIQNAEAVHFFC NYEESRGNYLVDVDGNRMLDLYSQI SSVPIGYSHPALLKLIQQPQNASMF VNRPALGILPPENFVEKLRQSLLSV APKGMSQLITMACGSCSNENALKTI FMWYRSKERGQRGFSQEELETCMIN QAPGCPDYSILSFMGAFHGRTMGCL ATTHSKAIHKIDIPSFDWPIAPFPR LKYPLEEFVKENQQEEARCLEEVED LIVKYRKKKKTVAGIIVEPIQSEGG DNHASDDFFRKLRDIARKHGCAFLV DEVQTGGGCTGKFWAHEHWGLDDPA DVMTFSKKMMTGGFFHKEEFRPNAP YRIFNTWLGDPSKNLLLAEVINIIK REDLLNNAAHAGKALLTGLLDLQAR YPQFISRVRGRGTFCSFDTPDDSIR NKLILIARNKGVVLGGCGDKSIRFR PTLVFRDHHAHLFLNIFSDILADFK TRTRPLEQKLISEEDLAANDILDYK DDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
