| Cat.No. : |
ACE2-026H |
| Product Overview : |
Recombinant human ACE-2, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques. |
| Description : |
The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. ACE2 is known to be expressed in various human organs, and its organ- and cell-specific expression suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronavirus HCoV-NL63 and the human severe acute respiratory syndrome coronaviruses, SARS-CoV and SARS-CoV-2, the latter is the causative agent of coronavirus disease-2019 (COVID-19). Multiple splice variants have been found for this gene and the dACE2 (or MIRb-ACE2) splice variant has been found to be interferon inducible. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
hIgG/His |
| Form : |
Liquid |
| Bio-activity : |
Measured by its binding ability in a functional ELISA with SARS-CoV-2 Spike RBD. The ED50 range ≤ 100 ng/mL. Specific activity is > 250 pmol/min/μg, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of Mca-YVADAPK(Dnp)-OH per minute at pH 7.5, at 25 centigrade. |
| Molecular Mass : |
110.8 kDa |
| AA Sequence : |
<DGS>QSTIE EQAKTFLDKF NHEAEDLFYQ SSLASWNYNT NITEENVQNM NNAGDKWSAF LKEQSTLAQM YPLQEIQNLT VKLQLQALQQ NGSSVLSEDK SKRLNTILNT MSTIYSTGKV CNPDNPQECL LLEPGLNEIM ANSLDYNERL WAWESWRSEV GKQLRPLYEE YVVLKNEMAR ANHYEDYGDY WRGDYEVNGV DGYDYSRGQL IEDVEHTFEE IKPLYEHLHA YVRAKLMNAY PSYISPIGCL PAHLLGDMWG RFWTNLYSLT VPFGQKPNID VTDAMVDQAW DAQRIFKEAE KFFVSVGLPN MTQGFWENSM LTDPGNVQKA VCHPTAWDLG KGDFRILMCT KVTMDDFLTA HHEMGHIQYD MAYAAQPFLL RNGANEGFHE AVGEIMSLSA ATPKHLKSIG LLSPDFQEDN ETEINFLLKQ ALTIVGTLPF TYMLEKWRWM VFKGEIPKDQ WMKKWWEMKR EIVGVVEPVP HDETYCDPAS LFHVSNDYSF IRYYTRTLYQ FQFQEALCQA AKHEGPLHKC DISNSTEAGQ KLFNMLRLGK SEPWTLALEN VVGAKNMNVR PLLNYFEPLF TWLKDQNKNS FVGWSTDWSP YADQSIKVRI SLKSALGDKA YEWNDNEMYL FRSSVAYAMR QYFLKVKNQM ILFGEEDVRV ANLKPRISFN FFVTAPKNVS DIIPRTEVEK AIRMSRSRIN DAFRLNDNSL EFLGIQPTLG PPNQPPVS<L EPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GKHHHHHH> |
| Endotoxin : |
< 1 EU/μg of protein (determined by LAL method) |
| Purity : |
> 90% by SDS-PAGE |
| Applications : |
SDS-PAGE, Enzyme Activity, Bioactivity |
| Storage : |
Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -105 centigrade. Avoid repeated freezing and thawing cycles. |
| Concentration : |
0.5 mg/mL (determined by Absorbance at 280nm) |
| Storage Buffer : |
Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol |
| SDS-PAGE : |
3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain
|
| Biological Activity : |
SARS-CoV-2 Spike RBD is coated at 5 μg/mL (100 μL/well) can bind Human ACE-2. The ED50 range ≤ 100ng/mL.
|
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.