Species : |
Human |
Source : |
Sf9 Cells |
Tag : |
His |
Description : |
Human α3 chain of collagen IV; identical with the antigen called "glomerular basal membrane antigen" (GBM). Recombinant antigen for solid (ELISA) and fluid phase diagnostic assays. Binds IgG-type human auto-antibodies. The term minicollagen designates the removal of most of the epitope-less triplehelical collagenous region (situated between the N-terminal 7S domain and the C-terminal noncollagenous NC1 domain), which is a requirement for recombinant production of this antigen. |
Coating Concentration : |
0.12-0.5 μg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination. |
Applications : |
Standard ELISA test; Immuno-Dot test with positive/negative sera panels; SDS-PAGE; Western-Blot. |
Caution : |
It has been reported that the immunodominant epitope of GBM is a cryptic epitope that is not easily accessible to the corresponding autoantibodies. It is necessary to treat the protein under nonreducing conditions with a denaturant such as urea to unmask the epitopes (see Hellmark et al. in Autoantibodies, Peter, J.B. and Shoenfeld, Y., eds., Elsevier B.V., 1996, pp 291-298). |
Storage & Stability : |
Recommendations for storage buffer: ion strength 100mM, neutral to slightly alkaline pH and 20 % glycerol as cryoprotective agent. Storage temperature: –70° to –80° C. Please prevent freeze-thaw cycles. |