Active Recombinant Human AGRN Protein, His-tagged

• Cat.No.: AGRN-652H
• Product Overview: Recombinant Human AGRN(Thr30-Arg1102) fused with His tag at C-terminal was expressed in CHO.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: Thr30-Arg1102
• Predicted N Terminal: Thr30
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons on a Laminin alpha 4 coated plate. Able to significantly inhibit neurite outgrowth when immobilized as a 3 μL droplet containing 50 ng of protein.
• Molecular Mass: Predicted Molecular Mass: 113 kDa;
  SDS-PAGE: 138-181 kDa, reducing conditions.
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE with silver staining.
• Storage: Avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.

Gene Information

• Gene Name: AGRN agrin [ Homo sapiens ]
• Official Symbol: AGRN
• Synonyms: AGRN; agrin; AGRIN; agrin proteoglycan
• Gene ID: 375790
• mRNA Refseq: NM_198576
• Protein Refseq: NP_940978
• MIM: 103320
• UniProt ID: O00468