Recombinant Human ANGPTL3, His-tagged

• Cat.No.: ANGPTL3-101H
• Product Overview: Total 223 AA. MW: 26 kDa (calculated). N-Terminal His-tag, 16 extra AA (highlighted).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is predominantly expressed in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain and the C-terminal fibrinogen (FBN)-like domain.
• Form: Filtered (0.4 μm) and lyophilized in 0.5 mg/mL in 0.05M Acetate buffer pH 4.
• Purity: >95%
• Research topic: Energy metabolism and body weight regulation.
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Applications: Biologically active protein; ELISA; Western blotting
• Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein
• Sequences of amino acids: MRGSHHHHHH GMASHMSRID QDNSSFDSLS PEPKSRFAML DDVKILANGL LQLGHGLKDF VHKTKGQIND IFQKLNIFDQ SFYDLSLQTS EIKEEEKELR RTTYKLQVKN EEVKNMSLEL NSKLESLLEE KILLQQKVKY LEEQLTNLIQ NQPETPEHPE VTSLKTFVEK QDNSIKDLLQ TVEDQYKQLN QQHSQIKEIE NQLRRTSIQE PTEISLSSKP RAP
• Storage: Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.
• Pathways: Integrins in angiogenesis

Gene Information

• Gene Name: ANGPTL3 angiopoietin-like 3 [ Homo sapiens ]
• Official Symbol: ANGPTL3
• Synonyms: ANGPTL3; angiopoietin-related protein 3; FHBL2; ANGPT5; ANG-5; angiopoietin 5; OTTHUMP00000010719; UNQ153/PRO179
• Gene ID: 27329
• mRNA Refseq: NM_014495
• Protein Refseq: NP_055310
• MIM: 604774
• UniProt ID: Q9Y5C1
• Chromosome Location: 1p31.1-p22.3
• Function: cell surface binding; enzyme inhibitor activity; growth factor activity; integrin binding; phospholipase inhibitor activity; protein binding