Recombinant Human ANGPTL7 Protein, His-tagged

• Cat.No.: ANGPTL7-092H
• Product Overview: Recombinant human ANGPTL7 protein with His tag was expressed in HEK293.

Specification

• Description: Enables identical protein binding activity. Involved in negative regulation of vasculature development involved in avascular cornea development in camera-type eye and regulation of extracellular matrix organization. Located in extracellular region.
• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized
• Molecular Mass: 39 kDa
• Protein length: 346
• AA Sequence: MLKKPLSAVTWLCIFIVAFVSHPAW LQKLSKHKTPAQPQLKAANCCEEVK ELKAQVANLSSLLSELNKKQERDWV SVVMQVMELESNSKRMESRLTDAES KYSEMNNQIDIMQLQAAQTVTQTSA DAIYDCSSLYQKNYRISGVYKLPPD DFLGSPELEVFCDMETSGGGWTIIQ RRKSGLVSFYRDWKQYKQGFGSIRG DFWLGNEHIHRLSRQPTRLRVEMED WEGNLRYAEYSHFVLGNELNSYRLF LGNYTGNVGNDALQYHNNTAFSTKD KDNDNCLDKCAQLRKGGYWYNCCTD SNLNGVYYRLGEHNKHLDGITWYGW HGSTYSLKRVEMKIRPEDFKP
• Purity: > 98%
• Applications: WB; ELISA; FACS; FC
• Stability: This bioreagent is stable at 4 centigrade (short-term) and -70 centigrade(long-term). After reconstitution, sample may be stored at 4 centigrade for 2-7 days and below -18 centigrade for future use.
• Storage: At -20 centigrade.
• Concentration: 1 mg/mL
• Storage Buffer: PBS (pH 7.4-7.5). Sterile-filtered colorless solution.
• Reconstitution: Reconstitute in sterile distilled H2O to no less than 100 μg/mL; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.

Gene Information

• Gene Name: ANGPTL7 angiopoietin-like 7 [ Homo sapiens (human) ]
• Official Symbol: ANGPTL7
• Synonyms: ANGPTL7; angiopoietin-like 7; angiopoietin-related protein 7; AngX; CDT6; angiopoietin-like protein 7; angiopoietin-like factor (CDT6); cornea-derived transcript 6 protein; dJ647M16.1; RP4-647M16.2
• Gene ID: 10218
• mRNA Refseq: NM_021146
• Protein Refseq: NP_066969
• MIM: 618517
• UniProt ID: O43827

Reviews

07/26/2022

This stability is particularly advantageous for long-term studies or experiments involving extended incubation periods.

08/03/2019

ts high level of consistency allows for precise measurements and accurate data interpretation, enhancing the reliability of my findings.

04/15/2018

It minimizes the risk of protein degradation and maximizes the effectiveness of the protein in various applications, such as cell signaling pathways or metabolic studies.

08/27/2016

The manufacturer's deep understanding of the protein and its applications enables them to promptly and effectively address any issues that may arise during my trials.

Q&As

What are the known interactions of ANGPTL7 with other proteins or molecules?

ANGPTL7 has been shown to interact with several proteins and molecules. For example, it can interact with integrins, which are cell adhesion receptors involved in angiogenesis. ANGPTL7 has also been found to interact with heparan sulfate proteoglycans, which are molecules that help regulate cell adhesion and signaling during angiogenesis. Additionally, ANGPTL7 has been reported to interact with certain extracellular matrix proteins, such as fibronectin and collagen. These interactions suggest that ANGPTL7 may have a role in modulating cell-matrix interactions during angiogenesis.

How does ANGPTL7 affect the blood vessel formation process?

ANGPTL7 can have both pro-angiogenic and anti-angiogenic effects, depending on the context and cellular environment. In general, ANGPTL7 promotes blood vessel formation by stimulating endothelial cell migration, proliferation, and tube formation. It can also enhance angiogenic sprouting by promoting the proliferation and migration of endothelial tip cells. However, ANGPTL7 can also negatively regulate angiogenesis by inhibiting the activity of certain pro-angiogenic factors, such as VEGF. The precise mechanisms through which ANGPTL7 regulates blood vessel formation are still being investigated.

How is the expression of ANGPTL7 regulated?

The expression of ANGPTL7 can be regulated by various factors and signaling pathways. It has been found that certain growth factors, such as vascular endothelial growth factor (VEGF) and transforming growth factor-beta (TGF-β), can stimulate ANGPTL7 expression. Additionally, transcription factors like hypoxia-inducible factor-1 alpha (HIF-1α) and forkhead box protein C1 (FOXC1) have been shown to regulate the expression of ANGPTL7.

Are there any drugs or therapies targeting ANGPTL7?

At present, there are no approved drugs specifically targeting ANGPTL7. However, research is underway to develop therapeutic strategies that modulate ANGPTL7 activity. This includes exploring small molecules, antibodies, or RNA-based approaches to inhibit or modify ANGPTL7 function. These potential therapies may have future applications in the treatment of angiogenesis-related disorders.

What are the future directions of ANGPTL7 research?

Future research on ANGPTL7 is likely to focus on further understanding its role in angiogenesis, as well as its implications in disease pathology. This may involve studying the molecular mechanisms by which ANGPTL7 regulates blood vessel formation and exploring its interactions with other angiogenic factors. Additionally, clinical studies may evaluate the potential of targeting ANGPTL7 for therapeutic purposes.

Is ANGPTL7 protein associated with any diseases?

Yes, ANGPTL7 has been implicated in various diseases and conditions. For instance, it has been found to play a role in the development of certain eye disorders like glaucoma and increased intraocular pressure. Additionally, altered expression of ANGPTL7 has been observed in different types of cancer, suggesting its involvement in tumor angiogenesis and progression.

Are there any therapeutic implications for ANGPTL7 protein?

Research on ANGPTL7 is ongoing, and it is being studied as a potential therapeutic target. Modulating the activity of ANGPTL7 may have therapeutic benefits in diseases characterized by abnormal angiogenesis, such as cancer and certain eye disorders. However, more research is needed to fully understand its potential therapeutic applications.

Are there any drugs or therapies targeting ANGPTL7 in development?

As of now, there are no approved drugs or therapies specifically targeting ANGPTL7. However, due to its involvement in angiogenesis and potential implications in various diseases, including cancer and ocular disorders, researchers are actively exploring the therapeutic potential of ANGPTL7. Studies have investigated the use of ANGPTL7 inhibitors or antibodies to block its pro-angiogenic effects in preclinical models. These efforts may lead to the development of targeted therapies aimed at inhibiting ANGPTL7's activity in specific disease contexts.

Are there any genetic mutations or polymorphisms associated with ANGPTL7 and disease susceptibility?

Genetic mutations or polymorphisms in the ANGPTL7 gene have not been extensively studied for disease susceptibility. However, a few studies have identified specific ANGPTL7 gene variants that may be associated with ocular disorders, such as primary open-angle glaucoma. These findings suggest that genetic variations in ANGPTL7 could potentially influence disease susceptibility or progression. More research is needed to fully characterize the genetic variations of ANGPTL7 and their impact on disease susceptibility.

Is ANGPTL7 involved in other biological processes besides angiogenesis?

While ANGPTL7 is primarily known for its role in angiogenesis, emerging evidence suggests that it may be involved in other biological processes as well. For example, studies have suggested a potential role for ANGPTL7 in wound healing and tissue repair. ANGPTL7 has also been implicated in the regulation of extracellular matrix remodeling and fibrosis. Furthermore, recent studies have linked ANGPTL7 to the regulation of lipid metabolism, indicating its involvement in metabolic processes beyond angiogenesis.

Can ANGPTL7 be used as a biomarker for disease diagnosis or prognosis?

ANGPTL7 has shown some potential as a biomarker for certain diseases. For example, increased expression of ANGPTL7 has been observed in the aqueous humor of glaucoma patients compared to healthy individuals, suggesting its potential as a diagnostic biomarker for this condition. Additionally, ANGPTL7 expression levels have been associated with poor prognosis in certain types of cancer, such as breast cancer and hepatocellular carcinoma. However, more research is needed to validate ANGPTL7 as a reliable biomarker and determine its clinical utility in disease diagnosis and prognosis.

Can ANGPTL7 be used as a diagnostic marker for cancer or eye disorders?

While ANGPTL7 shows altered expression in certain cancers and eye disorders, it is not currently used as a diagnostic marker. Diagnostic markers for such conditions are typically specific proteins or genetic mutations that are more reliable and validated. However, ongoing research may explore the potential utility of ANGPTL7 as a diagnostic or prognostic marker in these diseases.

Can ANGPTL7 be used as a therapeutic target for anti-angiogenic therapies?

ANGPTL7 has shown promise as a potential target for anti-angiogenic therapies. Its ability to modulate various aspects of angiogenesis makes it an attractive candidate for interventions aimed at controlling abnormal blood vessel growth in diseases such as cancer and ocular disorders. However, further research is necessary to fully understand the complexities of ANGPTL7's functions and interactions before developing therapeutic strategies targeting this protein.