Recombinant Human ApoE3

• Cat.No.: ApoE3-3562H
• Product Overview: ApoE3

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 317
• Description: Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants.
• Form: Lyophilized
• Molecular Mass: 36,154 Da
• Purity: >90% by SDS-PAGE & HPLC analyses
• Notes: Small volumes of ApoE3 recombinant protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
• Warning: This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Gene Information

• Gene Name: ApoE3
• Official Symbol: ApoE3
• Synonyms: APOE; APOE; AD2; LPG; LDLCQ5; Apo-E
• Gene ID: 348
• mRNA Refseq: NM_000041.2
• Protein Refseq: NP_000032.1
• MIM: 104310
• UniProt ID: P02649
• Chromosome Location: Chromosome: 19; NC_000019.10 (44905782..44909393). Location: 19q13.2
• Function: ?ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants

Citation

Imipramine and olanzapine block apoE4-catalyzed polymerization of Aβ and show evidence of improving Alzheimer’s disease cognition

Journal: Alzheimer's Research & Therapy    PubMed ID: 35768831    Data: 2022/6/29

Authors: Noah R. Johnson, Athena C.-J. Wang, Huntington Potter

Article Snippet:Once the optimal concentrations of approximately 20 μM Aβ42, 1 nM apoE, and 15 μM ThT were determined, they were maintained throughout subsequent studies unless noted otherwise.Once the optimal concentrations of approximately 20 μM Aβ42, 1 nM apoE, and 15 μM ThT were determined, they were maintained throughout subsequent studies unless noted otherwise.. For HTS assay validation, recombinant human Aβ40 (rPeptide), recombinant human scrambled Aβ42 (rPeptide), recombinant human apoE2 and apoE3 (Creative BioMart), recombinant human apolipoprotein A-I (apoA-I; Creative BioMart), human plasma-derived apoE (Sigma), or dimethyl sulfoxide (DMSO; Sigma) were included or substituted at the indicated concentrations.. In assay optimization experiments, 3?8 wells were used per group and experiments were replicated one or two times, as indicated in the figure legends.In assay optimization experiments, 3?8 wells were used per group and experiments were replicated one or two times, as indicated in the figure legends.